Hi all, After nearly a month of troubleshooting the fabrication of Whitesides Chamber we finally have the capability to generate a steady and linear gradient of molecules. In the presented case, we have generated a gradient of rhodamine 6G (R6G),…
Hi all, After nearly a month of troubleshooting the fabrication of Whitesides Chamber we finally have the capability to generate a steady and linear gradient of molecules. In the presented case, we have generated a gradient of rhodamine 6G (R6G),…
I was motivated to scan our membrane edges after looking at this image from the paper Maryna presented last week: I found a couple of pnc-Si samples with membranes still attached to the edges. I also found one sample with…
In this post I show the sieving coefficients of simulations with a thin membrane, a thick membrane, or a no membrane case as they approach equilibrium. The simulations were carried out for 48 hours, and plotted for 1 hour time…
Initial studies into the mechanical properties of pnc-Si were carried out using the “bulge test”. With JP’s help, we’ve collected a large amount of pressure-displacement measurements. This technique has been used to investigate thin film membranes suspended over windows of…
Here is the most up to date figure that I’ve made to compare the simulated and experimental separation results. I went back through the x-ray crystals and remeasured the longest distances in the molecules. I used the radii to rerun…
In this post I will explain how I tried to use the 1D diffusion model to check the results we had in the Nature paper. Unfortunately I didn’t get the same results in the simulation and I present some ideas…
In this post I will show that the analytical 1D model of diffusion is similar to our 3D COMSOL model, with the exception that the times do not seem to line up. This is reasonable though since the 1D model…
A temperature series was performed last week over seven wafers annealed inside the susceptor (15 nm silicon). The objective was to determine if the current configuration of target materials would show pore tunability over the temperatures of interest (900 –…
This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells. These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days. Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but…