In my previous post, I showed images of bEnd (P8) cells on PET and pnc-Si transwells.  These were seeded at 105 cells/cm2 and grown for 14 days.  This post contains the TEER data.  I simply normalized all values to the…

I continued to run some of the samples that I described in this post last week.  These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS.  The pnc-Si was SC126 that was RTP’ed. …

Using the same procedure as in the last post, I was able to simulate a 6um thick membrane with the same pore characteristics as the experimental pnc-Si membranes.  Material diffuses more slowly through this membrane, but not as slowly as…

Last week, we ran several wafers with a silicon target from Refining Systems (Las Vegas).  In addition to the source material being different, the target bond was a silver elastomer vs. the typical indium bond that AJA uses.  Below is…

Last week, Barrett and I made up the three buffers needed to perform this test: Dissociating buffer: 0.1 M glycine-HCl, pH 3.5 1 M Tris Binding buffer: 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.5 In addition, I made…

Follow up to this post.  As a brief reminder, I have performed 3-D simulations of separations in large and small volume systems.  I have defined an effective diffusion coefficient that takes into account the membrane hindrance and compared this to…

With the previous 2 experiments, I made a couple of 6-slit pnc-Si transwells.  These chips were from the cell culture wafers I had made a while ago.  These samples (SC126) were RTP’ed before use. My improved technique, combined with the…

Along with the PET data I just posted, I also examined cells on pnc-Si transwells.  The following images were taken at 20X (through the bottom of a 24-well plate), and the cells are passage 8 bEnd3.  They were seeded on…

A while ago, I posted some images of cells grown on commercial polyester (PET) transwells.  Here’s the post.  I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the…

Recently, SiMPore has been exploring alternate ways for depositing our film stack.  We’ve finished our initial testing of a plasma-enhanced chemical vapor deposited film by Rogue Valley (OR), and it appears that the pore formation process is inhibited with the…