Category: NRG

Diffusion Time Dependence

The following is a plot of rhodamine diffusion for the experimental system at various time point: In this figure, the rhodamine approaches equilibrium, as defined as a filtrate/retentate value of 1.  The 9x well chips have a larger active area…

Posted in NRG

pnc-Si bioreactor for red blood cell culture!

Hi all, this is Henry. Leeanna Hyacinth, an undergraduate student from the McNair program, has recently completed a joint project between the Waugh, Palis, and McGrath Lab to create a bioreactor to amplify the number of mature red blood cells.…

Posted in NRG

Electroosmosis problems

I am having some problems with my electroosmosis device and I was hoping that people with more knowledge about the TEM chips might have some suggestions.  For these trials, I used membranes from a wafer labeled 412-S.  I am still…

Posted in NRG

Modeling Diffusion with COMSOL

Due to the discrepancies between the 1D model and experimental runs with rhodamine, I have switched over to using COMSOL to solve for a 3D model.  In this system, 200×200 um membrane squares with a 45 degree etch separate the two…

Posted in NRG

Pore tunability, consistency

In the last month, we’ve run several sets of 15 nm pnc-Si wafers over a range of RTP temperatures.  Below is a chart that illustrates the current pore size control we have by tuning annealing temp. The 900 C, 950…

Posted in NRG

UV-Ozone treated SC267: DDM passes and BSA held back

Building on my recent posts I ran an experiment with SC267 testing the ability of BSA and DDM to diffuse through UV-Ozone treated (20min at 150C) and untreated samples. I used four samples testing both BSA and DDM in the…

Posted in NRG

Diffusion to equilibrium dependent on geometry

In order to further test the model, I’ve set up additional rhodamine diffusion assays.  In each case, the setup is similar to the nanoparticle/protein/dna separations –  20 uL of .5 mM rhodamine on the top of a SepCon and 20…

Posted in NRG

Dye Diffuses through UV-Ozone Treated and Untreated SC267 Samples

In my last post I presented the results of an experiment in which it appeared that BSA did not diffuse through a SC267 sample treated in the UV-Ozone system for 20min at 150C. As a control I also ran BSA…

Posted in NRG

More Modeling

Frustration with the lack of agreement with experimental device has led me to check if the diffusion in the wells within the model is physically relavent.  To do so I’ve solved a partial differential equation pertaining to a concentration of…

Posted in NRG

Diffusion of BSA through SC267 prevented using UV-Ozone

As mentioned in my previous few posts Justin and I have been working to characterize contact angles resultant from various exposures in the UV-Ozone system. Earlier this week we got our new oxygen tank installed and it appears that after…

Posted in NRG
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