In effort to understand the model, I have been studying simple diffusion experiments using rhodamine, a small molecule expected to experience little hindrance.  The model currently expects a small molecule the size of rhodamine to reach equilibrium within 1.5 hours.…

Continuing the project that Mark had been working on, I have made some progress towards electroosmosis. Specifically, I was able to observe electrolysis through an intact membrane. Hopefully I will be able to observe electroosmosis through the movement of microspheres…

This is an update of my progress with the diffusion model.   Sieving coefficients for standard setup (Renkin only) In this image there’s a strong sigmoidal curve for the 24 hour diffusion.  However, the cutoff (~34nm) is higher than cutoffs…

Last week I made a post showing the diffusion of DDM and BSA through SC267. I an effort to increase the negative charge associated with the membrane material I proposed using UV-Ozone exposure. A while back there was some concern…

The purpose of this post is to see if there is a pattern in the Pore Density vs. Pore Radius graphs at certain manufacturing conditions. The following wafers all have widths of 15nm and RTPs of 1000. The porosities range…

The goal of this rotation was to create a device to test the electro-osmotic properties of the membrane bound silicon chips. To perform this task, it was necessary to attach two glass pipettes onto both sides of a chip. The…

Last week I made a post showing detection of detergent diffusion across membrane SC267. In the diffusion set up I had placed 6uL of a 50/50 volume ratio solution of 1.0% DDM and 1mg/mL BSA with 60uL of DI-water on…

I’ve been thinking of demos/experiments to do with my upcoming class and one of them is SEM.  I was thinking of handing out some unknown nanoparticle solutions and have them figure out the size and maybe material by SEM, AFM…

Over the past few weeks, I have been trying to separate proteins with the SepCons. As a preliminary test, Barrett and I decided to first acertain that BSA could pass through a membrane with pinholes. Theoretically, the pinholes are so…