On Friday I posted results from a diffusion experiment in which I tested “treated” and “untreated” SC256 samples against the diffusion of a mixture of DDM and BSA. The DDM-BSA solution contained an initial DDM concentration of 1.0% and an…

In an effort to conserve membrane samples I am working to run diffusion experiments with a mixture of BSA and DDM. This will only use 2 chips instead of 4. In our meeting on Tuesday it was mentioned that BSA…

I’ve been attempting to simulate protein sieving using the COMSOL model.  This first figure shows the comparison of the simulated separations to the actual results as determined by gel densitometry. The simulated separations tend to lag behind the experimental data…

After our meeting with Professor Dumont on Tuesday it was decided that we needed to try and perform the BSA/DDM seperation with a more porous membrane (to allow for eventual pressurization). SC256 was choosen and as an initial step I…

The last post I made about the BSA/DDM separations using “treated” and “untreated” SC269 samples explained a problem I was having in which the initial retentate drop was diffusing around the edge of the sample chip. It seems that SC269…

Yesterday I posted results of a diffusion experiment using rhodamine and fluoroscein that showed the pores in SC269 are open both before and after treatment in the UV-Ozone system. I set up another diffusion experiment to test “treated” and “untreated”…

We haven’t talked much about the optical properties of pnc-Si, but I thought it would be good to share some of the index and dispersion data I’ve collected using the ellipsometer.  Below are plots of index of refraction (n) and…

This is a follow-up post to “Protein Assay Test.” I realized that I never posted the final stag of my experiments, in which I finally got BSA to go through the membranes. In “Protein Assay Test,” I described several experiments…

Yesterday I posted my results from a BSA diffusion experiment using “treated” and “untreated” SC269 samples. To ensure that the pores in SC269 are indeed open I took a step backwards and set up a diffusion experiment using small dye…

As I mentioned in the meeting this morning the SC267 samples have run out so I have begun testing SC269 for use in my experiments. This post pertains to a BSA diffusion experiment that I set up yesterday and allowed…