We now have a linear standard curve for sodium fluorescein (Na-F) in clear 24-well plates.  What is the detection limit? The detection limit is defined as the lowest background-corrected concentration that is 3 standard deviations above the noise of the…

Charles designed a macro for excel to represent the data gathered from wafer characterization, in a visual format. It is built on VB platform and runs off Microsoft excel. The data is input in a simple fashion by selecting the…

Now that we have preliminary transport data from the Tecan, we need standard curves in order to get permeability coefficients. This is really tricky because the RFU (relative fluorescent units) are arbitrary and based on the Tecan gain. For example,…

A study of membrane deflection has been conducted using the Veeco Wyco optical profiler. This is the first time the Veeco has been used to obtain quantitative data describing membrane deflection. This type of analysis will be useful for several…

The question arose last week about the affects of transport with use of the shaker in the Tecan, which led to a discussion about the current construction of the sepcon. The sepcon layout currently is as follows: The o-ring is…

All of this talk about Zeta Potentials, electro-osmosis and electric double layers got me thinking about these phenomenon in relation to discoloration.  We’ve known that salts accelerate discoloration, and we know that bicarbonate solutions accelerate discoloration as well.  I explained…

This is a follow-up to my last post about gold separations.  Using the Tecan, I’ve scanned the absorbance spectrum of all the gold particles we posses in water, 10 mM KCl, and 100 mM KCl.  Here are the results: The…

This is a follow up to Jess’ protein separation post.  She mentioned that some of the SC 073 samples were annealed twice: the first was a 700 C 60 s anneal to crystallize (before etching) and the second was a…

Two of the newer wafers, SC 073 and SC 074, came back with ~30 intact samples.  This would be more than enough for the separation work that I’m trying to finish, so I decided to see if they were any…

Last week, I ran a few experiments to see how certain membrane conditions affected the transport of fluoroscein. The first experiment presented here contained three sepcons: one with 3 pinholes, one with only 1 intact slit, and another without slits…