This is a follow-up to my last post about gold separations.  Using the Tecan, I’ve scanned the absorbance spectrum of all the gold particles we posses in water, 10 mM KCl, and 100 mM KCl.  Here are the results: The…

This is a follow up to Jess’ protein separation post.  She mentioned that some of the SC 073 samples were annealed twice: the first was a 700 C 60 s anneal to crystallize (before etching) and the second was a…

Two of the newer wafers, SC 073 and SC 074, came back with ~30 intact samples.  This would be more than enough for the separation work that I’m trying to finish, so I decided to see if they were any…

Last week, I ran a few experiments to see how certain membrane conditions affected the transport of fluoroscein. The first experiment presented here contained three sepcons: one with 3 pinholes, one with only 1 intact slit, and another without slits…

The highly anticipated 3rd generation etch cell has arrived.  NY Manufacturing did a great job with the design and fabrication.  To give an idea of how far we’ve come, I’ve placed previous etch cells next to the newest. One of…

The AJA sputtering system has 2 oxide targets and 1 silicon target that are focused with stainless steel chimneys.  During our process, films inevitably deposit on the chimney and shutters and after a while start cracking causing flakes of material…

A long running side story to my research has been attempted separations of nanogold. The basic idea is that we want to separate EM quality gold nanoparticles with the membrane, but I have had problems detecting gold in the filtrate…

This post is a continuation of the experiments posted a couple of days ago. In that post, I concluded that absorbance measurements of molecules transported through transwells were not possible if the transwell insert was kept in the 24-well during…

Here is the excitation and emission wavelengths for two common fluorophores for transport: rhodamine and fluoroscein. rhodamine fluoroscein max ABS 480 nm 470 nm emission 558 nm 516 nm excitation 524 nm 492 nm The protocol for finding excitation and…