There have been differences between the previous long term discoloration studies carried out by myself and Barrett. Couple of reasons speculated for the differences were: 1) Wafer variation (w683 vs w704); w683: has no visible pores, is more amorphous in…
Tag: discoloration
Maximum stability in cell culture conditions
This experiment was carried out to determine the maximum life of pnc-Si in cell culture conditions based on discoloration. All the discoloration slowing factors were put together to see how long would the membranes last for. These include: samples in…
Discoloration Array (revisited)
I repeated Barrett’s discoloration array experiment (Array1, Array2). NaCl solution was prepared @ concentrations: 0, 0.25 (0.1), 0.5 and 1M, whereas NaHCO3 solution was prepared @ 0, 0.475, 0.95, 1.90 (and 3.80) mg/ml. Different combinations of both these salt solutions…
RTP + Silanization (3D format)
As seen before, in 2D format ‘RTP+silanization’ helps. Similarly in 3D format, with membrane side down, the same treatment helps prevent discoloration till 5 days. (samples from W413) Above figure shows how combined effect of RTP and silanization is better…
Pore processing of DMEM incubated samples
In continuation of the previous post, I ran the pore processing software for two samples (t=0 and t=150 min). The following histograms show the pore parameters and the difference in the pore size and numbers between the two samples. I…
TEM images of pnc-Si incubated in DMEM(-FBS)
There has been uncertainty with process of dissolution of pnc-Si in cell growth media. Whether the media enters the pores and ‘eats away’ the material leading to increase in pore sizes and eventually complete degradation was the question. Thinning of…
RTP + Silanization
After recent studies with surface treatments, we wanted to take a step further by trying amino silanization on RTP’ed samples. Wafer 413 samples were RTP’d with no intact slits. Both 2D and 3D formats were tested in DMEM (-FBS) in…
Liquid Silanization again
A bunch of samples from W683 (no RTP) were liquid silanized and used in 3D format to track discoloration in comparison to control sample, in DMEM(-FBS) @ 37C in Oven. I used all the cracked chips which broke while clamping…
Silanization and BSA coating
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in…
BSA coating on pncSi (3D vs 2D)
I carried out a repetition of BSA coating experiment with both 2D and 3D formats. With 2D, I get the same result as before, but surprisingly, in Sepcon 3D format we observe the opposite to what we see in 2D!…
