This post explains that electroosmosis in our material is higher than we expected. Jim suggested that maybe we don’t have fully developed flow in our thin material. Here I attempt to find the entrance length, or distance until fully developed…

I recently completed a HUVEC adhesion study with commercial PET and pnc-Si transwells.  HUVEC were seeded @ 100000 cells/cm^2, allowed to attach for 5 hours (upside down format) and then stained with CMFDA.  Images were acquired without autocontrast, but I…

Hi all, After a brief hiatus from winter break and my paper writing Mike and i have resumed our work on fabricating the counetrflow system, and we do have a success. Fabrication: The counterflow system is constructed based on oxide-layer…

In an attempt to push the cutoff higher, we annealed two wafers outside the susceptor at 1000 C (SC 066) and 1100 C (SC 067) with a ramp rate of 100 C/s. The 1100 C does give a cutoff slightly…

Below are a series of TEM micrographs showing a set of membranes produced last week.  We RTPed these wafers at 700 (SC 062), 800 (SC 064) and 850 C (SC065) without the susceptor at a ramp rate of 100 C/s.…

This is kind of a shot in the dark, but I wanted to see if I could get a zeta potential for pnc-Si that was as high as predicted by the electroosmosis results. First I tried to get the membranes…

As I told at the meeting, I tried spincoating PDMS on the membrane and it worked without breaking membrane. To make an ultrathin polymer film by spincoating the polymer has to be diluted in the particular solvent first. I used…

Since HUVEC morphology hasn’t looked good in short-term adhesion assays recently, I analyzed HUVEC on the 2nd day after cell seeding. Normally, CMFDA staining is done for 30-45 minutes in serum-free media.  I wondered if the serum-free environment during staining…