After four days of this ongoing HUVEC culture in pncSi transwell, HUVECs look much better than before. Here I have freshly stained them with Calcein AM and we can see how bright it is in comparison to CMFDA stained cells…
Category: NRG
Production updates
There’s been a lot of production in the last month and we’re still sorting through the data, but here is a synopsis of some key findings. All films were deposited with the AJA tool. Film reproducibility Week-to-week variation in pore…
Posted in NRG
Day2:Cell Adhesion (Trial 3)
On Day 2 of cell growth from trial 3 of adhesion study, cells looked much more spread out but less in density. Most of the cells were observed to have concentrated near the edge of the pnc-Si chip near the…
Cell Adhesion (Trial 3)
Samples (no RTP) from W673 and W684 were taken (four from each wafer; with atleast one blank chip) in Sepcons and HUVECs were plated @ ~19,000 cells/sq. cm. density. Cells were allowed to adhere for over 4 hours and this…
Contact Angle Study
According to recent cell adhesion studies on aminosilanized pncSi, no significant difference between adherence to the silanized and non-silanized surfaces was found. This could have been due to the adsorption of protein from the cell growth media onto the surface…
HUVEC adhesion – high cell seeding densities
This was my first experiment in which the Sepcons were inverted for cell adhesion. After allowing the cells to attach for 4.5 hours, I inverted the Sepcons in media for staining with CMFDA. Therefore, the Sepcon is set up so…
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Cell Adhesion Study (Trial 2)
I repeated the previous experiment, this time plating slightly higher density of cells: ~15100 cells/sq.cm. Samples were from the same W673, no RTP) After counting the cells stained using Calcein AM, % Adhesion w.r.t the cells plated were found to…
Electroosmotic Theory
This is something that’s been living on my knowledge page, but I think it’s a good idea to put it up here to get some discussion on it. Probstein gives the following equation for electroosmotic velocity in his book Physicochemical…
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Cell Adhesion Study (on silanized surface)
We have seen before that silanization slows down discoloration and hence prevents membrane degradation. In Sepcon, the sample should last over a week. Further, it was necessary to check whether silanized surface supports normal cell growth. Therefore, comparison of cell…
AFM-Pore Processing analysis of Discoloration
This is a continuation of the last post on AFM analysis of pore changes during discoloration. I took the AFM images, inverted them in ImageJ so that the pores would be white and then did PoreProcessing in MATLAB. I simply…
Posted in NRG
