Just to double check the numbers I calculated on Tuesday I ran some wet-wet trials with 100K and 300K Nanoseps. Based on the wet-dry results we would expect that the permeability for the 100K Nanosep would be higher than that…
Category: NRG
UV Ozone treatment of membranes
UV Ozone treated W404 sample testing (benchtop) : In EGM In L15 In DMEM In each row, the first two samples (from left) are UV Ozone treated whereas the next two are not.It can be observed that there is no…
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Membrane in Sodium Chloride solution
W407 (non treated) in different NaCl salt concentrations (benchtop) : At t=0 In order (from l-r)1. 0.5 M NaCl2. 1.0 M NaCl3. 2.0 M NaCl At t=24 hrs. At t=53 hrs. At t=6 hrs and t=12 hrs the chips were…
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Membrane characteristics (w404 vs. w336)
Membrane stabilityNon treatedW404 samples in DMEM 15um beads on the membrane:15um beads below the slit:In order: (left to right)1. t=02. t=33. t=64. t=105. t=13 hrs.Non treated W336 samples in DMEM15um beads on the membrane:15um beads below the slit:In order: (left…
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Updated 100K microcon wet-wet permeability
A while ago I made a post about the permeability through the 100K microcons in the wet-wet EQ format. I had run three membranes, and stopped (and restarted) the centrifugation at three separate time points to be able to calculate…
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Burst Pressures and Air Permeability after Ozone treatment
I tested ozone treatment influence on the membrane strength and air permeability. The result: didn’t see any difference between ozone treated and non-treated samples. Burst pressure experiments description: tried wafers 391, 393, 344, and 345. Ozone treatment for 5 and…
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Membrane collapse in culture media
Above video shows the disruption of the membrane from W404 after around 12 hours in the DMEM media (benchtop). The membrane was not treated with UV-Ozone and degraded pretty early as compared to that from W336 which broke at ~28…
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UV/Ozone cleaner operation reminder
Maryna told me the UV/ozone cleaner did not work (the blue light in front of the chamber did not light up) when she was working with Dave today. I quickly checked the machine and it is actually working properly. The…
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Transmission Spectra of pnc-Si
UPDATE:Chris took the membrane sample and normalized with by the no spanning membrane sample. This was plotted against the theoretical spectrum of single crystal silicon. The curves have a similar shape, but the single crystal silicon has lower transmission. This…
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Permeability and Ozone Treatment
I treated samples from w312 using our ozone equipment. Quickly, here was the protocol:Place samples in chamberTurn on O2 and supplemental Ozone generatorAfter 3 mins, turn on Lamp in main chamber for 5 mins (Heater set to 110C)Leave samples in…
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