We have recently been discussing the best way to analyze the diffusion and SepCon separation gels. In the following figures, I present line scans of the gels. These appear to be a bit noisy and are somewhat misaligned due to…

Using wafer 302 I set up 2 20nm and 2 50nm gold diffusion experiments. After 24 hrs I removed the retentate and filtrate and looked for the gold using the Malvern Zetasizer. We can’t perform the diffusion with both 20nm…

Last week we processed wafers 335 – 340. Our goal was to track down the source of Cu contamination. We sputtered membrane films both at room temperature and at 400 C. Our thought was that the RT dep. would not…

There is some non-uniformity in air permeability based on the position but it is not very clear for wafers 318 and 319. The full wafer measurement in future will make this dependence more obvious.Permeability of w319 is much higher then…

The 300K nanosep membranes that Jess ordered came in so I ran a single set of three membranes at 21.63psi to compare their hydraulic permeability values to those of the 100K microcon membranes. As expected the 300K nanosep membranes have…

Having finished the hydraulic permeability testing with DI water I wanted to see what the effect of an introduction of protein would have on the permeability values. To do this I simply reran a set of fresh membranes at 21.63psi…

When I first starting testing the microcon filters I noticed that if they were allowed to sit overnight (exposed to air) after having been already tested with water the next day if they were tested again the permeability of the…

Last week, I took some of Jess’ 20 nm Au colloids and placed them on a membrane for imaging under TEM. I took images at each of our standard magnifications. Using Mike B.’s pore counting program, I generated statistics and…

My previous post outlined the results of a EQ format separations using membranes from wafer 319. I have followed up that experiment using the same membranes, but in a diffusion based separation. I separated 2uL of 9.3 mg/mL clarified brain…

There’s been some discussion on whether furnace-annealed-only wafers are more uniform than RTP’ed ones. Today, I looked at several samples from wafer 304 (FA 1000 C) at different positions along the y-axis. (0, 5) – 25kx (0,2) – 25kx (0,1)…