The 300K nanosep membranes that Jess ordered came in so I ran a single set of three membranes at 21.63psi to compare their hydraulic permeability values to those of the 100K microcon membranes. As expected the 300K nanosep membranes have…

Having finished the hydraulic permeability testing with DI water I wanted to see what the effect of an introduction of protein would have on the permeability values. To do this I simply reran a set of fresh membranes at 21.63psi…

When I first starting testing the microcon filters I noticed that if they were allowed to sit overnight (exposed to air) after having been already tested with water the next day if they were tested again the permeability of the…

Last week, I took some of Jess’ 20 nm Au colloids and placed them on a membrane for imaging under TEM. I took images at each of our standard magnifications. Using Mike B.’s pore counting program, I generated statistics and…

My previous post outlined the results of a EQ format separations using membranes from wafer 319. I have followed up that experiment using the same membranes, but in a diffusion based separation. I separated 2uL of 9.3 mg/mL clarified brain…

There’s been some discussion on whether furnace-annealed-only wafers are more uniform than RTP’ed ones. Today, I looked at several samples from wafer 304 (FA 1000 C) at different positions along the y-axis. (0, 5) – 25kx (0,2) – 25kx (0,1)…

We finally achieved a separation using the EQ format. 4 SepCons from wafer 319 were loaded with 500 uL of .93 mg/mL clarified brain extract. They were run at 1000 rpm (which we roughly figured to be 3.3 psi initially)…

Here are the conversion ratios for the URMC TEM images (so we’re all using the same standard): 30kx – 0.39 px/nm50kx – 0.67 px/nm150kx – 2.2 px/nm300kx – 4.5 px/nm These numbers should be used for wafers 187, 189, and…

Dave and I tested 8 wafers (187, 189, 310, 316, 318, 325, 327, 329) from the last 3 runs and traces of copper can be found within all the wells. However, the amount and position of this contamination varies greatly,…

This is re-do of a post I made before the new year regarding the predictions of the ‘Tong equation’ for wafer 310 (I deleted the earlier post because I came to the wrong conclusion). I wrote MATLAB files to calculate…