We finally achieved a separation using the EQ format. 4 SepCons from wafer 319 were loaded with 500 uL of .93 mg/mL clarified brain extract. They were run at 1000 rpm (which we roughly figured to be 3.3 psi initially)…

Here are the conversion ratios for the URMC TEM images (so we’re all using the same standard): 30kx – 0.39 px/nm50kx – 0.67 px/nm150kx – 2.2 px/nm300kx – 4.5 px/nm These numbers should be used for wafers 187, 189, and…

Dave and I tested 8 wafers (187, 189, 310, 316, 318, 325, 327, 329) from the last 3 runs and traces of copper can be found within all the wells. However, the amount and position of this contamination varies greatly,…

This is re-do of a post I made before the new year regarding the predictions of the ‘Tong equation’ for wafer 310 (I deleted the earlier post because I came to the wrong conclusion). I wrote MATLAB files to calculate…

Here is a look at all our water permeability through nanomembranes to-date. There appears to be subtle trends showing increasing permeability with radial position (r – values) and a not-so-subtle trend showing decreasing flow with protein (cyt and albumin). Note…

Wafer 302 appears to have no pinholes and held up pretty well in the centrifuge, up to 5psi for many hours in a wet-wet format. Here are some quick numbers on flow rate over 2 hours, 3.3psi, including radial position:1x…

I derived the governing equation for the volume transfer from SepCon devices in the wet/wet (EQ) format. It is … where the equilibrium volume for the cup can be calculated apriori using the cross sectional areas of the cup and…

This is probably not of general interest, but for the sake of full disclosure, I have spent considerable time working with our wafer supplier (Virginia Seiconductor) to resolve some issues regarding the surface quality of their wafers. It would have…

After the w310 series being beautiful (few pinholes, reasonable strength, high yield), we have retreated somewhat with a return of the copper-related piholes. Here is an SEM image and EDX scan from the well wall: Here is a pic of…

SepCon EQ reaches Equilibrium in 2 1/2 hours!w310 (no pinholes)1x PBS (no protein)2 hours @ 1000rpm (3.3psi) -> 103 uL passage (56 uL/(min-cm2-psi) )2 1/2 hours @ 1500 rpm (5 psi) -> 204 uL passage (>74 uL/(min-cm2-psi) ) In the…