Previous work on Tomograms: TEM Tomography of NPN and npMgF2 Previous work on reconstructing the tomograms: Manual Reconstruction of Electron Tomograms Introduction This post examines another set of MgF2 pores that Kilean acquired. I want to compare the inner pore morphology of two…
Category: NRG
Bonding SiMPore Chips Directly to Glass has been Difficult
Our friends at McGill university have wanted to incorporate our chips directly into their platform for squeezing and confining DNA. Their desires come in a form of a device where we can seal the SiMPore chips into glass channels without…
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Variation of TEER on the active area and membrane geometry
[Background read – https://trace-bmps.org/blog/theory/2015/01/28/why-teer-values-are-multiplied-by-membrane-area-dc-version] I am putting the excerpt of this old post from Jim in this post. Tom helped me in gathering this data. In order to prove my point that electrical resistance is very sensitive to the…
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Beckham Lab Exosome Ultracentrifugation Protocol
When Aslan and I went to the Beckham lab last week, they gave us a copy of their ultracentrifugation protocol for isolating exosomes. I attached it here so that we can have it as a reference. The major thing to…
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ADSC Mechanotransduction: YAP/TAZ, adhesion complexes, and the cytoskeleton
YAP/TAZ YAP/TAZ intracellular localization, and subsequent transcriptional activity, has indicated its role in pathways of mechanotransduction. Investigation of the effects of substrate stiffness on MSC growth and differentiation point to YAP/TAZ as an indicator of how cells are perceiving their mechanical…
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Summary for Visiting the Urology lab
Hello Everyone, Here is an update of our meeting in Strong Hospital: So They are working in an urology lab, We met Sam who is a technician in Dr. Carla Beckham’s lab, he is responsible for cell culture part of…
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SEM 4 slot 75 nm NPN
SEM of lot # 1208, four-slot 75-nm NPN at 1014x, 1972x, 4101, and 5351x mag. The next image is with a tilted sample. (from the same lot of chips #1208 4-slot 5.4 mm chips) The next five show the edge…
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Manual Reconstruction of Electron Tomograms
Using the techniques described in my previous post: TEM Tomography of NPN and npMgF2, we have recreated some tomograms that have contour (position) and height (depth) information contained in them. Our goal now is to express that height information in the…
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TEM images of dried gold solutions
For the modeling paper, I had to precisely quantify the distribution of gold nanoparticles in the BBI solutions. To do this, I dried the stock solutions of particles on TEM grids (I don’t know what chips I used, but I seem…
RPE ZO-1 expression observed on MgF2 and tissue culture plastic
Here are some more images from my ongoing cell culture project. My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates I added twice the amount of primary antibody on these devices that I usually do (1:100…
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