Over the course of this past year and 3 months, I have been on a long and perilous quest to reproduce data that I obtained during my rotation in the McGrath Lab. Specifically, I was looking to capture exosomes in…

Hey all, Here’s my thesis: ksmith thesis I filmed the public portion of my defense and posted the video to youtube. I’m not leaving till the end of the week, and I’ll be back for a week in mid-May as well…

Hello everyone, this post is a summary/recap of the MRS Spring meeting that was held in Phoenix, Arizona this past week (April 17 – 21). Here is an overview of two sessions: Molecular and colloidal plasmonics – Synthesis and applications…

The x-values are based on TEM images of the last populations of gold nanoparticles (I think. I can’t remember immediately when the fridge died on us). It’s probably worth re-doing that with the new gold vials. There’s still some work…

Previous work on Tomograms: TEM Tomography of NPN and npMgF2 Previous work on reconstructing the tomograms: Manual Reconstruction of Electron Tomograms Introduction This post examines another set of MgF2 pores that Kilean acquired. I want to compare the inner pore morphology of two…

Our friends at McGill university have wanted to incorporate our chips directly into their platform for squeezing and confining DNA. Their desires come in a form of a device where we can seal the SiMPore chips into glass channels without…

[Background read – https://trace-bmps.org/blog/theory/2015/01/28/why-teer-values-are-multiplied-by-membrane-area-dc-version] I am putting the excerpt of this old post from Jim in this post.   Tom helped me in gathering this data. In order to prove my point that electrical resistance is very sensitive to the…

When Aslan and I went to the Beckham lab last week, they gave us a copy of their ultracentrifugation protocol for isolating exosomes. I attached it here so that we can have it as a reference. The major thing to…

YAP/TAZ YAP/TAZ intracellular localization, and subsequent transcriptional activity, has indicated its role in pathways of mechanotransduction. Investigation of the effects of substrate stiffness on MSC growth and differentiation point to YAP/TAZ as an indicator of how cells are perceiving their mechanical…

Hello Everyone, Here is an update of our meeting in Strong Hospital: So They are working in an urology lab, We met Sam who is a technician in Dr. Carla Beckham’s lab, he is responsible for cell culture part of…