SEM of lot # 1208, four-slot 75-nm NPN at 1014x, 1972x, 4101, and 5351x mag. The next image is with a tilted sample. (from the same lot of chips #1208 4-slot 5.4 mm chips) The next five show the edge…

Using the techniques described in my previous post: TEM Tomography of NPN and npMgF2, we have recreated some tomograms that have contour (position) and height (depth) information contained in them. Our goal now is to express that height information in the…

Here are some more images from my ongoing cell culture project. My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates I added twice the amount of primary antibody on these devices that I usually do (1:100…

[presentation] HUVEC migration and fibronectin

I will keep this post short and start by saying that the process of establishing a reliable set-up for nanosphere deposition, by evaluating the repeatability of the deposition parameters, has been longer than anticipated. The nanospheres self-assemble at the hexane-water…

Many, many thanks to Kilean Lucas and Brian McIntyre who enabled our metrology. We have desired to get 3d information about the structure of our nanopores. One method to do so is known as electron tomography, a technique which can…

Two weeks ago (as of the time of writing this post), I wrote a post on a dead end filtration experiment that I ran in order to demonstrate particle capture in pores, modeling a simple, purified exosome capture system that…

-Intro The mechanobiology behind endothelial cells has been extensively studied in recent years. Shear stress as well as substrate stiffness have both been shown to influence endothelial cell signaling and morphology in vitro [1,2]. However, the combination effect of both shear stress…

This is the second in my series of posts that will be on my model exosome system. As I am trying to define the appropriate conditions for separating exosomes from blood and other fluids, I am first going through and…