Photo Courtesy J. Adam Fenster, U of R We’ve had several of each of the two prototype reverse Sepcon designs professionally 3D printed for us (top photo). I did a few quick tests to see how they worked in the…
Category: NRG
Rotation Project: The Influence of Collagen Gels and Nanoporous Chips on Shear Based Endothelial Cell Alignment
As an introduction, my name is Alec Salminen. I received my BS in Biomedical Engineering at the University of Arkansas and am currently in my first year of the BME PhD program at the University of Rochester. As my rotation…
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Rotation Project Complete: Dialysis of cytochrome c across a commercial membrane
Dan Steiner Rotation Summary Abstract The purpose of this rotation project was to develop and test a clampable system for eventual use in scalable nanomembrane dialysis systems. The nanomembrane dialysis system will be miniaturized into a compact, wearable hemodialysis device that…
MANUSCRIPT REVISION: Membrane pore spacing can modulate endothelial cell-substrate and cell-cell interactions
Membrane pore spacing can modulate endothelial cell- substrate and cell-cell interactions (REVISION) We submitted our “focal adhesions” paper to ACS Biomaterials Science and Engineering late last year. We received reasonable reviewer requests from three reviewers. I think I am mostly…
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NanoAuger Analysis of Magnesium Fluoride Nanomembranes: The Hunt for Silicon Nitride
This is my third post that is related to the NanoAuger system that I had the opportunity to use while I was home over break. However, instead of quantum dots and exosomes, this time I will be talking about data…
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Continuation on Quantifying SiteClick Membrane Efficacy
The purpose of this experiment was to quantify the effectiveness of the kit membrane at separating free antibody and qdots from qdot-bound antibodies. The manual advertises an approximate 80% yield for this step and no purity reading. It was originally…
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Exosome Identification: Quantum Dot Labeling and NanoAuger Analysis
Alright, so this is kind of a big update and I will probably split it into a couple of parts. Basically, in a search for a way of confirming that the particles we are seeing on the membranes, I have…
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Auger Electron Spectroscopy: A Basic Overview
Since I am posting data that is acquired using Auger electron spectroscopy, I thought that it might be a good idea to write a post about the theory of the instrument, to better understand why this data is useful and…
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Microporous MgF2 Burst Pressure Fabrication and Results
Summary Statement: We have doubled our MgF2 yields (compared to nanoporous MgF2) by moving to thicker microporous membranes. While the increase in strength is more modest than initially expected, we have plenty of substrates for use in cell culture. Statistics: Window…
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Ultracentrifugation Exosome Controls: An Unusual Observation in Separation
In attempting to generate control systems for our exosome work as well as to potentially develop a system that would help some collaborators at the University of Illinois, I have been working with the ultracentrifuge again to hopefully isolate a…
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