The blog has migrated to a new hosting provider. This means that the passwords for owncloud and the blog are no longer in sync. Owncloud has been upgraded from 5.0.18 to 7.0.4. The passwords have not been changed and are…

I want to verify that I can grow a confluent monolayer of cells on my MgF2 nanomembranes. I have tried to do this in the past, but have only gotten sparse cells overall before the membranes ruptured. I believe that…

This is more work that Randi did looking at ADSCs migrating towards HUVEC networks.   Motivation: To demonstrate the migration of ADSC toward pre-formed HUVEC networks when ADSC are seeded 4 hours after HUVEC. Method: Monday – Coat 4 wells…

There are now two ways to sign up to receive new blog content by e-mail. The original way was disabled for a few months, but is now back. This is the approach that creates a beautiful e-mail with full post…

I’ve been trying for a year now to make a nanofluidic transistor – a nanomembrane with a thin layer of metal coated in dielectric that allows for the dynamic modulation of the sieving characteristics of the chip. Beginning with the…

All separations are 30 nm (diameter) gold nanoparticles in a 56 nm pore setup, with other parameters as described in my last post. Y-axis scale bar is in meters, x-axis scale is meaningless (the simulation is 1-D, and the width…

I have wondered if it were possible to create free-standing, nanoporous nanomembranes out of other materials (rather than my MgF2 process). The process is general enough that it should work. The major factors are Etch Selectivity between the substrate and…

These curves were all generated using the following package of matlab functions and scripts: (Sieving copy – run Find_Sieving_Coefficient_boltzmann.m – it’s set up to generate concentration profiles right now). The program begins with a ‘standard separation’ that uses the following characteristics: Pore diameter:…

After a great deal of debugging and thought, I’ve made a small breakthrough in the modeling of sieving behavior using MATLAB, Bill Deen’s electrostatic model of pore-particle interactions, and a boltzman distribution to model concentration polarization (previous blog posts: Part…

We wanted to prove our previous findings that ADSC crawl toward HUVEC networks and alter them. We did this by using time lapse imagery to demonstrate the migration. We also wanted to show the process of HUVEC network formation. We…