Testing was performed on 3D-printed transwell device prototypes for use as culture devices with SEPCON chip integration(Makerbot2 printed Transwells for SEPCON chips). The performed testing attempted to determine the quality of seal of interfaces between all device components. The two…
Category: NRG
Permeance tests with new Omega rotameter
Yaron has acquired an Omega rotameter (FL-3803ST) with interchangeable flow tubes. The tubes operate in three different ranges low (6-61 mL/min, 042-15-N), medium (150-1,682 mL/min, 082-03-N), and High (1,000-23,752 mL/min, 034-39-N). I’ve attached an excel file with the calibration data…
Makerbot2 printed Transwells for SEPCON chips
In Nottingham this summer, I attempted to use a Makerbot2 printer to create culture devices for SEPCON chips. I had varying degrees of success, but I was not able to get a good seal between the PLA and gaskets using…
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NP + IgG cleanup in 0.1% Tween 20
Our previous work with reverse centrifugation NPN membranes has shown that they may work for cleaning up excess protein from conjugated NPs. This initial success used ~20 nm red fluospheres with membranes from wafer 1070 (10-15%, ~45nm) and unlabeled IgG…
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STTR: Silicon Nanomembranes for Ab-labeled NP clean-up
Submitted on April 7, 2014. Posted to NRG Cloud under NRG/Funding. Figure 2. Dead End Filtration with SEPCONTM (A) SiMPore’s SEPCONTM spin cup with a silicon nanomembrane chip. B) Dead end filtration in a centrifuge. The direction of the centrifugal…
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Concept for proposal to NIST
I am planning to write a proposal to the NIST Measurement Science and Engineering (MSE) Research Grant Program. Federal Funding Opportunity (FFO) is here. The proposal is due in the beginning of June. From talking with a friend at RIT would…
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Surfactants tested to reduce agglomeration between IgG and 20 nm fluospheres
Mary Lee and I performed a quick test to determine the efficacy of different surfactants at reducing agglomeration between IgG and 20 nm fluospheres. Each solution is a combination of 50 uL 10 mg/mL IgG (in PBS) + 50 uL…
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Endothelial Vacuolization Induced By Highly-permeable Silicon Membranes
Submitted to Acta Biomaterialia – March 15, 2014 Manuscript as PDF Check Status
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NanoDays 3/29 & 3/30
We still need help with NanoDays http://doodle.com/paq66krxtf7mt6tk There are two time slots: 10:45am-2pm 1:45-4pm Please wear your I Love Nano shirts from last year. I have a couple more size Medium and one size Small. The Rochester Museum & Science…
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XPS data on oxidized and surface treated NPN
Katia Lyubarskaya in Alex Shestopalov’s lab has been testing different surface conditions of oxidised NPN membranes. I’ve placed her data below. (We used chips from wafer 1070) We started by looking at chips that received different chemical/plasma/thermal processes to…
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