Ultimately we want to use SiN-NP filters to separate viruses or some other biological species, but repeated attempts to pass 20 nm fluorospheres in water or PBS have failed.  Au particles, however, have passed through the filters.  This post reports…

Following up on our previous work with SiN-NP with varied RIE times, Sarah and I measured permeability and permeance using a new measuring system better suited to the higher permeance and permeability we expect with the larger pores and higher porosity.…

JP and Josh Miller used the Xactic Si etcher to remove the pnc-Si from SiN-NP samples.  I believe they ran cycles until the samples looked visibly like SiN only.  Below are some SEM cross-sections showing before and after images of…

The following is a public service announcement: I’ve just aliquoted about 40mL of fluorescent BSA and placed it in the freezer. Each aliquot contains roughly 1mL of 6mg/mL TRITC-conjugate BSA, so there’s plenty of them. If anyone needs fluorescent BSA for anything,…

As I mentioned in my last post, I will be performing protein diffusion tests through CytoVu assemblies (from one well to the other) and measuring the amount of protein that is still in solution when the system reaches equilibrium in…

This is a follow up to Mohammad’s previous posts: “Burst Pressure”, and “Burst Pressure Probability”. Josh and I had Mohammad’s wafer remade, and burst several 30nm thick pnc-Si TEM chips to add to Mohammad’s earlier data. Pore size still needs to…

I’ve performed what might be the last test of the n-Dodecyl β-D-Maltoside (“DDM”) coating. This time I washed for three minutes each sample in HBS just like in the reference, used precisely the same concentration (and type, for that matter) of…

AFM images report Protocol: pnc-Si TEM  grids were mounted on thin silicone inserts. Surface treatments were applied to the grids. Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids …

I’ve been using a Makerbot 2 for the last week or so, trying to see if we can use the same PDMS contact lithography techniques that we are all familiar with. In short, yes, but there is plenty of work…