Sarah and I did some more gold separations with 10, 20, 40, 50, 60, 80 and 100 nm Au particles.  We used the “clean” water purchased from the medical center.  Same as last time, we diluted the “as-bought” gold 3…

Our work with permeance and hydraulic permeability tests on the SiN-NP seemed to show that the porosity and pore sizes I had calculated from the STEM images were too high.  I used URnano’s TEM to get better quality images and,…

I found another old wafer # 760 and asked Josh Miller to deposit a 25-50-25 OSO stack.  He did a standard RTP of 1050 C, 60 s, 100 C/s.  (and Josh Miller did the EDP etch, so this is really…

Since the basic protein diffusion experiments went well, I’m moving up to something that more closely resembles an actual assay application. The basic idea is this: Biotin-conjugate FLAG antibody will be bound to Avidin acrylic beads in order to make…

Ultimately we want to use SiN-NP filters to separate viruses or some other biological species, but repeated attempts to pass 20 nm fluorospheres in water or PBS have failed.  Au particles, however, have passed through the filters.  This post reports…

Following up on our previous work with SiN-NP with varied RIE times, Sarah and I measured permeability and permeance using a new measuring system better suited to the higher permeance and permeability we expect with the larger pores and higher porosity.…

JP and Josh Miller used the Xactic Si etcher to remove the pnc-Si from SiN-NP samples.  I believe they ran cycles until the samples looked visibly like SiN only.  Below are some SEM cross-sections showing before and after images of…

The following is a public service announcement: I’ve just aliquoted about 40mL of fluorescent BSA and placed it in the freezer. Each aliquot contains roughly 1mL of 6mg/mL TRITC-conjugate BSA, so there’s plenty of them. If anyone needs fluorescent BSA for anything,…

As I mentioned in my last post, I will be performing protein diffusion tests through CytoVu assemblies (from one well to the other) and measuring the amount of protein that is still in solution when the system reaches equilibrium in…

This is a follow up to Mohammad’s previous posts: “Burst Pressure”, and “Burst Pressure Probability”. Josh and I had Mohammad’s wafer remade, and burst several 30nm thick pnc-Si TEM chips to add to Mohammad’s earlier data. Pore size still needs to…