Now that I’ve got plenty of fluorescent protein, I’ve moved back to testing protein adsorption to various surfaces. I’ve repeated the n-Dodecyl β-D-Maltoside (“DDM”) coating procedure (reference) with the goal of preventing or heavily decreasing the protein adsorption to the PDMS…

The overall goal was to see is if it was possible to create a reliable low resolution mask that can be used for crosslinking PEG structures using a laser printer and transparencies sheets. With these masks, I explored to see…

I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them.  From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included:…

I made this post last week concerning testing this procedure to make sure that the resulting solution is in fact fluorescent BSA and not just a mixture of free BSA and free TRITC. Unfortunately, I exercised my right as a rookie…

In my previous post, I mentioned that the EA treated chips showed decreasing contact angles after their storage in water in contrast to gel-pack or vacuum container. However the EA treatment was 4 days old, and I lacked adequate controls.…

IMPORTANT: I made a mistake, and mistook what was actually Tetramethylrhodamine-5-Iodoacetamide Dihydroidiode (5-TMRIA) for TRITC. 5-TMRIA is another Rhodamine derivative and has similar chemical properties to TRITC, but does not bind to proteins in the same way. The procedure should…

Silver chloride electrodes are useful for microfluidic devices since they can generate current below one volt. This allows them to function without fear of electrolysis confounding experimental results by way of gaseous clogging. Several different methods for coating silver mesh…

Presentation from yesterday: PPT, PDF

In this trial, bEnd.3 cells were seeded onto three single-well CytoVu devices with SiO2 membranes coated with a 1% Geltrex coating.  After ~27 hours, the cells were fixed and permeabilized (the fixing may be only partial though, as the solution…

Karl and attempted to pass 10, 20, 40 and 50 nm Au NPs through samples from the center of wafer # 1019 (avg por dia 50-60 nm, porosity ~27%).  The bottles of stock Au from BBI solutions do not have…