Continuing on the path of hemodialysis, we are increasing the complexity of the fluid being dialyzed. In this long-term experiment I will pump 100% serum with added urea (1mM) cytochrome c (1 mg/mL) and fluorescent BSA (1 mg/mL). So I…
Category: NRG
PDMS Coating Issues
I’ve performed what might be the last test of the n-Dodecyl β-D-Maltoside (“DDM”) coating. This time I washed for three minutes each sample in HBS just like in the reference, used precisely the same concentration (and type, for that matter) of…
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AFM Scans of Coated, Annealed TEM Windows
AFM images report Protocol: pnc-Si TEM grids were mounted on thin silicone inserts. Surface treatments were applied to the grids. Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids …
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3D Printing for Milli/Microfluidics
I’ve been using a Makerbot 2 for the last week or so, trying to see if we can use the same PDMS contact lithography techniques that we are all familiar with. In short, yes, but there is plenty of work…
Posted in NRG
Take Two: n-Dodecyl β-D-Maltoside Coating on PDMS
Now that I’ve got plenty of fluorescent protein, I’ve moved back to testing protein adsorption to various surfaces. I’ve repeated the n-Dodecyl β-D-Maltoside (“DDM”) coating procedure (reference) with the goal of preventing or heavily decreasing the protein adsorption to the PDMS…
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PEG Structures with Masks from Laser Printer
The overall goal was to see is if it was possible to create a reliable low resolution mask that can be used for crosslinking PEG structures using a laser printer and transparencies sheets. With these masks, I explored to see…
Posted in NRG
Single-Well CytoVus w/ SiO2 Membrane – Comparisons
I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them. From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included:…
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For real this time: BSA-TRITC conjugation procedure works!
I made this post last week concerning testing this procedure to make sure that the resulting solution is in fact fluorescent BSA and not just a mixture of free BSA and free TRITC. Unfortunately, I exercised my right as a rookie…
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Changes in hydrophilicity of EA treated pncSi after storage in water
In my previous post, I mentioned that the EA treated chips showed decreasing contact angles after their storage in water in contrast to gel-pack or vacuum container. However the EA treatment was 4 days old, and I lacked adequate controls.…
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Procedure for labeling BSA with TRITC works
IMPORTANT: I made a mistake, and mistook what was actually Tetramethylrhodamine-5-Iodoacetamide Dihydroidiode (5-TMRIA) for TRITC. 5-TMRIA is another Rhodamine derivative and has similar chemical properties to TRITC, but does not bind to proteins in the same way. The procedure should…
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