Category: Protocols

Makerware 3d print Preview

One neat feature of the Makerware software is to visualize the printed layers before actually printing them. You can catch a lot of errors with this feature; I highly recommend it.

Posted in Protocols

Importing Gasket Geometries to Silhouette from Sketchup

We currently use Adobe Illustrator to design silicone gasket geometries for our microfluidic devices. Here is a quick way to do a similar thing from sketchup. The advantage of this procedure is that you can create a complete 3d model of

Posted in Protocols, Protocols (Public)

Animations with Sketchup

Sketchup is a great tool for showing off your microsystem ideas, complete with animation capabilities. However, it cannot intrinsically move objects around, show how water flows in a channel, or  insert pieces into a slot, which are useful for understanding

Posted in Protocols

Permeance tests with new Omega rotameter

Yaron has acquired an Omega rotameter (FL-3803ST) with interchangeable flow tubes.  The tubes operate in three different ranges low (6-61 mL/min, 042-15-N), medium (150-1,682 mL/min, 082-03-N), and High (1,000-23,752 mL/min, 034-39-N).  I’ve attached an excel file with the calibration data

Posted in NRG, Protocols

SOP for bonding of PDMS to NPN

I’ve written a quick SOP document reflecting the procedure that resulting in successful permanent PDMS-NPN bonding. The procedure described in this document has not been optimized and may include unnecessary or otherwise sub-optimal steps. These steps reflect the steps that WERE

Posted in Protocols

Small Animal Model Hemodialysis Set-Up

Sprague-Dawley rats will be purchased from Harlan. These pre-manipulated animals will have been catheterized prior to shipment. We will feed them an adenine rich diet to induce uremia, high levels of urea in the blood. Once uremic, a rat will

Posted in Protocols Tagged with: ,

Schematic of Long Term Clearance Test Setup.

This is the setup used for the long term clearance testing. The fraction collector can be set to advance to the next collection tube at set intervals from 1 min to 99 min. I am currently conducting an evaporation test

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BBB Device 2.7 Fabrication with Mortar Bonding

Fabricate ITO Electrodes Deposit 2500 Angstroms, ITO, Room Temperature Using Kurt Lesker Sputterer (Use ITO Variable Recipe, takes about 1.5hr) Tape in Microscope Slides and Coverglass into Machined Shadow Mask using Kapton Tape Anneal in Toaster Oven, 450 Fahrenheit, 1hr

Posted in Protocols
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