I assembled two 2-chip dialyzers using the thicker, 75-nm NPN membrane chips (10 mm long multichannel, 22 mm x 24 mm). The dialyzers were pumped full of IPA, to fill with fewer bubbles, then infused with PBS for at least…
Tag: Dean
Adsorption Test of PEG4, IgG and BSA
Discussion: The BSA may have not been fresh enough to give good data, the difference between background and signal are pretty low. The Rhodamine IgG gave better signals but the PEG4 treated SiN is between the untreated SiN and bare…
Urea Clearance: First Animal Study Results
BOTTOM LINE: We had great results from the small number of nanoporous membranes used to clear urea from Sprague-Dawley rats. We are clearing Urea better than commercial materials. (see fig) Recap of previous work: First we leaned how to set…
Small Animal Model Hemodialysis Set-Up
Sprague-Dawley rats will be purchased from Harlan. These pre-manipulated animals will have been catheterized prior to shipment. We will feed them an adenine rich diet to induce uremia, high levels of urea in the blood. Once uremic, a rat will…
Review of ACKD nanotechnology articles (Vol 20, issue 6, Nov 2013)
This is a cursory review of the nonotechnology articles appearing along with our paper in the Journal “Advances in Chronic Kidney Disease” Most of these are reviews and most deal with nanoparticles for either imaging or therapy. One does discuss…
Hemodialysis in the rat animal model
I found some good descriptions of hemodialysis experiments using rats. They all use a method possibly first described by (Rockel et al., 1978). (Galons, Trouard, Gmitro, & Lien, 1996) at U of Arizona used nephrectomized male Spraque-Dawley rats (Holtzmann Co.,…
Hemo biometrics for various animals
In determining an appropriate animal model for hemodialysis efficacy, several metrics need to be compared to the human vasculature. Blood volume, blood pressure, and heart rate could effect the suitability of these animals to model clinical hemodialysis. Blood volume can…
Urea/f-BSA/cytochrome c concentrations in retentate after dialysis: 12 hours
Continuing on the path of hemodialysis, we are increasing the complexity of the fluid being dialyzed. In this long-term experiment I will pump 100% serum with added urea (1mM) cytochrome c (1 mg/mL) and fluorescent BSA (1 mg/mL). So I…
Long Term Urea: Evaporation measurement and Pressure Calculations.
The initial measurements with multiple tests tubes containing either Serum or PBS failed to demonstrate evaporation and in fact the weights of the collection tubes increased after sitting in the refrigerator overnight. Perhaps the tape collected water? At any rate…
Schematic of Long Term Clearance Test Setup.
This is the setup used for the long term clearance testing. The fraction collector can be set to advance to the next collection tube at set intervals from 1 min to 99 min. I am currently conducting an evaporation test…
