The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which…
Tag: desalting
Bradford Assay to Quantify Protein Retention During Desalting
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which…
Desalting with Intact Membranes: Continued
As mentioned previously intact membranes show a flux comparable to membranes with pinholes during desalting experiments. To confirm this a third intact membrane test and two pinholed tests were run under the exact same conditions. Previous pinholed samples had different…
Desalting: Continued Work
In continuation of the desalting work, I ran three additional controls: a SepCon with no slits (negative control) and direct addition of 4M NaCl producing final dilutions of 1:400 and 1:600 (these are the experimental final dilutions I have been…
Desalting: Broken and Pinholed Membranes
Desalting of 4M NaCl in deionized water was performed using our membranes in the SepCon format. I ran the SepCons in the same as way as the previous study, using a low stir rate and floating the SepCons. Once again,…
Desalting with Dialysis Cassettes
One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Two dialysis products made my Thermo/Pierce were tested to develop a protocol and test manufacturer’s claims. These products…
