EDIT: This problem was solved thanks to Tejas. It turns out that Falcon BD transparent 96 well plates absorb in the UV range, and are best used for bradford assays or anything that measures absorbance higher than the UV range.…
Tag: karl
Burst Pressure, Permeance of NSN pnc-Si SEPCONS with large pores
Karl, Charles and I measured the burst pressure and permeance of some 30-50-30 and 30-100-30 NSN. Burst Pressure: The SEPCONs readily burst at pressures under 1 psi when the flow was directed from the backside of the chip through the…
NSN SEPCONs
Still trying to reproduce Joe’s large pore material on SEPCONs using NSN stacks. This post has data from two wafers with 30/50/30 and 30/100/30 NSN. RTP is 1000C 50C/s for 1min. Nitride is etched off by placing samples in 50%…
Update: 30/100/30 OSO, 30/75/30 & 30/100/30 NSN SEPCON
We are trying to reproduce the very small pores Joe created with 30/100/30 OSO and large pores created with 30/100/30 NSN using a SEPCON pattern. (Joe’s previous post.) We had a problem with several chips releasing from the center of…
SEM Images of Barcikowski Samples
With Joe’s help, I was able to take some SEM images of the samples that I performed my previous set of separation experiments on. Sample 1: The obvious aggregation in this sample (exemplified by the ‘victory eagle’ blot in the…
Posted in NRG Tagged with: Barcikowski, clumping, dried sample, gold, karl, particles, platinum, SEM
Neither Centrifugation, Sonication, nor Filtration Fix the Aggregation in the Barcikowski Lab Samples
In early May, the Barcikowski lab shipped us four samples of gold nanoparticles for us to run separations on. They took forever to get here, and had aggregated and precipitated by the time they arrived. About a week ago, the…
High salt concentrations (~50mM) shouldn't be used to tune effective pore sizes for gold colloid separations
What we’d like to have accompany the Sepcon filters is a general way to tune the pore sizes for any given separation. If, for instance, someone wanted to have a filter cutoff of 12nm, we could create a custom wafer…
W613 Discoloration
Given the mixed results of discoloration of RTPed samples from W613, which include no discoloration until 7 days (when the experiment was started within 2 hours of RTPing), and discoloration within 2 days (experiment started 8 days after RTP) I…
Desalting BSA: Blank pnc-Si and Mini-Dialysis Units
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which…
BP Does Not Change with Membrane Orientation in Test Apparatus for W660
Given the low burst pressures of recently produced wafers Chris requested I burst pressure test some samples in two different orientations: well side facing away from the positive pressure source and well side facing towards the pressure source. The standard…
