Introduction The capability of the m-μSiM for establishing an hiPSC-derived blood-brain barrier (BBB) in bioengineering and bioscience laboratories has been shown previously [1]. To further demonstrate interlaboratory agreement and successful dissemination of components and protocols, m-μSiM was used at RIT…
Author: Mehran Mansouri
Gene expression analysis of HUVECs cultured on uSiM (static vs flow)
Introduction Studies have shown that the shear-stimulation of endothelial cells results in the upregulation of the shear-sensitive transcription factor Kruppel-like factor 2 (KLF2). KLF2 plays a critical role in the regulation of endothelial activity such as the regulation of vascular…
A Modular vascular barrier platform with a removable flow module and high resolution imaging (Publication draft)
1. Introduction 1.1 Vascular barriers Functions: Maintain tissue homeostasis, control transport and gas exchange, recruit immune cells during inflammation Figure 1. Schematic illustration of a vasculature barrier in the human body. Endothelial cells as a building element of vasculature barriers…
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New design of the flow platform with a functional lid (V2)
Introduction In a previous post, an initial version of the flow module and its sealing components were described. In order to improve the initial version based on collaborators’ feedback, several modifications have been made to simplify the device assembly for…
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Introduction of beads into the flow module
1. Introduction Fluid flow is one of the key elements of vascular models for delivering nutrients and growth factors to cells as well as introducing other components like neutrophils. In post 1, we described our modular design for incorporating a…
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Simulation and PIV results of the flow module
1. Introduction Fluid flow is an influential component of vascular models for conditioning cells and introducing other components such as leukocytes. In the previous post, we described our modular design for integrating a flow module into the uSiM device. Here,…
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Design and fabrication of the microfluidic flow module
Introduction Sepsis is a serious condition where unregulated systemic inflammation can cause organ failure and death. Recent evidence has also shown that sepsis can cause cognitive impairment in surviving patients. However, the mechanisms that cause sepsis-related damage to the brain…
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