Karl and I have been working to measure separations using SEPCONs with particles ranging in size from 20 nm to 1 µm. We started out naively enough by slightly diluting some flourospheres we found in the refrigerator, putting them in the…
Category: NRG
BBB V2.8 @ Vaccinex Adhesion Molecule Testing
I created some quick open air devices to test the viability of different adhesion molecules. The basal well has a volume around 150 uL, and the apical well has a volume around 15 uL. I made 6 devices in…
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Proposed time-dependent PEG treatment studies
In my previous post talking about the contact angle measurement of 3-mer coated PEG, there was a lack of adequate controls, and also an unanswered issue of contamination of samples during storage. I would now like to refine the study…
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PDMS Connector without capillaries
I found this article about a macro-microfluidic interconnect that I want to try out at some point. We might even be able to seal tube-block interface with PDMS for a tighter seal. http://blogs.rsc.org/chipsandtips/2006/11/13/a-simple-interface-between-a-pdms-microfluidic-device-and-a-syringe/ A simple interface between a PDMS…
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BBB Device V2.8 @ Vaccinex First Seeding Attempts
I made a lot of 6 devices for Vaccinex. We attempted to seed human endothelial cells on the membranes, flowing at 20 uL/min, first coating the surface of the membrane with an adhesion molecule at 20 uL/minute. At the same…
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Summary of Fissell's review paper in 2013 International Kidney
Quick review of “Achieving more frequent and longer dialysis for the majority: wearable dialysis and implantable artificial kidney devices” by Fissell, Roy, and Davenport see Fissell(Roy)_2013_International_Kidney.pdf in the hemodialysis section of the library. First a little background: Two type of…
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Notes regarding the PDMS syringe needle stamp
My past devices that utilize a membrane to separate particles have been haunted by mysterious pieces of junk. The other day I took a moment to inspect the holes that are punched using a sharpened 16ga needle in…
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550-600 nm diameter pores in 120 nm thick SiN
Wafer # 4369 (PFI 02) was fabricated with pore sizes ranging from ~550 – 600 nm. Below are two SEM images of the pores, followed by two STEM images. The pores are all open and very well defined. The only…
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Viscosity vs. [BSA]
Are you wondering why Dean has yet to go beyond 30% BSA in his clearance studies? Here is why … APP-04_Viscosity-of-BSA-in-PBS.pdf
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Cytochrome C polymerization
We have often encountered cytochome C dimers as part of our work. For example in this figure from Jess that we often refer to in our hemodialysis work. It turns out that Cyto C polymerization is a well known phenomenon.…
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