I made a lot of 6 devices for Vaccinex. We attempted to seed human endothelial cells on the membranes, flowing at 20 uL/min, first coating the surface of the membrane with an adhesion molecule at 20 uL/minute. At the same…

Quick review of “Achieving more frequent and longer dialysis for the majority: wearable dialysis and implantable artificial kidney devices” by Fissell, Roy, and Davenport see Fissell(Roy)_2013_International_Kidney.pdf in the hemodialysis section of the library. First a little background: Two type of…

    My past devices that utilize a membrane to separate particles have been haunted by mysterious pieces of junk. The other day I took a moment to inspect the holes that are punched using a sharpened 16ga needle in…

Wafer # 4369 (PFI 02) was fabricated with pore sizes ranging from ~550 – 600 nm.  Below are two SEM images of the pores, followed by two STEM images.  The pores are all open and very well defined.  The only…

 Are you wondering why Dean has yet to go beyond 30% BSA in his clearance studies? Here is why … APP-04_Viscosity-of-BSA-in-PBS.pdf

We have often encountered cytochome C dimers as part of our work. For example in this figure from Jess that we often refer to in our hemodialysis work. It turns out that Cyto C polymerization is a well known phenomenon.…

Hello everyone! The new FEI Osiris TEM system has been finally setup recently. With the new purchased Gatan single tilt heating holder 628, we were managed to do an in-situ heating experiment with the Osiris system last week. We used…

Power Point for my Senior Design   https://docs.google.com/file/d/0B1AH05-mf_tQLTdSMHRWX0NjNGc/edit?usp=sharing  

This is my first attempt of depositing PEG using vapor deposition methods, in a controlled fashion. The stepwise procedure: Josh was extremely kind by providing me with a diced pncSi wafer [windowless]. He taught me how to do the buffered…

A control was done to observe cell proliferation on a TPM coated cover glass. Images were taken at 10x after 4 days.  TPM coated cover glass  Polystyrene petri dish We are having some success with the seeding of bend.3 cells…