A new user in URnano has used the “old” susceptor for a ~270 C (I might have the exact number wrong, but it is relatively low temp) to heat In on GaAs. I don’t think there was ever a good…
Category: NRG
PEG Microarray Solution Dilution and X-linker Concentration Study
Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and…
Posted in NRG
Long Term Urea Clearance in 100% Serum
I ran a 30 hour urea clearance with 100% serum through the single slot dialysis device. The now standard protocol was run. The single slot chip was annealed in the RTP for robustness then clamped into the acrylic/PDMS flow fixture.…
Process Flows
What process flows have I used to create my devices? Standard Cut out gaskets (10 min) Embed Electrode (face down) (3 hrs) Ozone Bottom, Interstitial, Filter and Top channels together (1 hrs, cure overnight) Ozone Bottom Electrode (1 hr, cure…
Posted in NRG
Microscopy on TEM Grids with Kevin Webb @ University of Nottingham
Dr. Webb is very interested in the thin nanomembranes that our group experiments with. To date, he has been trying to get cells to grow on SiN microporous material and image them with a few different types of microscopy (Phase,…
Posted in NRG
Using Alumina Deposition to Modulate Pore Size
The Cambridge Microsystems Savannah 200 atomic layer deposition (ALD) system uses a two-step chemical process that alternates pulses of water vapor and trimethylaluminum (TMA) to build a layer of Al2O3 on a silicon substate. Because of the two-step nature of…
Fluorosphere wash and measurement with Tecan and Fluorescent microscope
Karl and I have been working to measure separations using SEPCONs with particles ranging in size from 20 nm to 1 µm. We started out naively enough by slightly diluting some flourospheres we found in the refrigerator, putting them in the…
BBB V2.8 @ Vaccinex Adhesion Molecule Testing
I created some quick open air devices to test the viability of different adhesion molecules. The basal well has a volume around 150 uL, and the apical well has a volume around 15 uL. I made 6 devices in…
Posted in NRG
Proposed time-dependent PEG treatment studies
In my previous post talking about the contact angle measurement of 3-mer coated PEG, there was a lack of adequate controls, and also an unanswered issue of contamination of samples during storage. I would now like to refine the study…
Posted in NRG
PDMS Connector without capillaries
I found this article about a macro-microfluidic interconnect that I want to try out at some point. We might even be able to seal tube-block interface with PDMS for a tighter seal. http://blogs.rsc.org/chipsandtips/2006/11/13/a-simple-interface-between-a-pdms-microfluidic-device-and-a-syringe/ A simple interface between a PDMS…
Posted in NRG
