Category: NRG

Pre-spring lab clean! (and o-rings)

Let’s get a blast-from-the-past and go through a bunch of drawers in the lab!  I spoke with Jim and it seems like it might be best to schedule it during next regularly slotted Tuesday NRG mtg.  So let’s plan on

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Cytochrome C can pass through 10nm EA-treated Sepcons

Using the remaining 10nm EA-treated chips (all of the 15nm EA-treated and all of the PEG-lyated chips had either been used or broken) I was able to pass cytochrome C through Sepcons. As usual, yields were low (~8-12uL) but the

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Simple PEG crosslinking on a microscope

We have re-started the PEG x-linking project at RIT. One of the goals is to create microwells made of PEG on top of a membrane in order to culture individual cells or small colonies of cells with the potential for

Posted in NRG

Substrate bias

We wanted to test the effects of changing the substrate bias during the Si deposition step similar to what Dave Fang had done so many years ago.  Attached is a couple of plots showing the effect of varying substrate bias

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If you do any separations with ultrathin nanoporous membranes please read this…

I’m not sure how many people in the current group have read all the papers that we have published, but a critical one was a JACS paper from Shigeru Amemiya where he used his Scanning ElectroChemical Microscopy (SECM) to probe

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BSA does not pass through Peg-lyated or EA treated Sepcons with cutoffs of either 10 or 15nm

  On Wednesday we ran several separations using both the EA and PEG treated membranes. The treatment process had damaged many of the chips. Of the 10 treated 10nm PEG chips, 2 survived. Of the 10 treated 15nm PEG chips,

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Vapor based PEG deposition

This paper talks about PEG monolayer deposition using CVD. Here is the link for Ultrathin Poly(ethylene glycol) Monolayers Formed by Chemical Vapor Deposition on Silicon Substrates  

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Hemodialysis update – Feb. 2013

Here is an aggregate of the recent posts/data on the Hemodialysis Project … Device Design/Demo Design idea for assembling multiple chips Gasket design for counter flow dialysis chip Peclet chip demo – > Dialysis Membrane modifications to prevent protein binding (HD

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shear stress reduction via pnc-Si: analytical solution REVISITED

Hi all, A while back (in the link below) I have posted the analytical solution that allows us to solve the flow rate distribution in our shear-free system. shear stress reduction via pnc-Si: analytical solution However we do not have

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Long Term Urea Clearance in Serum

I made 50 mL of 30% fetal bovine serum (FBS), 70% PBS, 0.5 mM urea. Pumped with syringe pump at 5.6 µL/min through an untreated and EA treated single slot dialysis chips in a stirred beaker of PBS (two separate

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