Some time before I noticed that placing the membrane in the metal deposition chamber at slight angle to the incoming metal atoms stream makes thin metal layer only on one side of the pore, shadowing the rest of it. Rotating…

My goal for this experiment was to see whether vacuoles form in primary cells.  If they did, this makes the case that the results with bEnd3 are more physiologically relevant since primary cells are better models of in vivo behavior. …

I wanted to confirm my results from this previous post.  Here, I used lower concentrations of bafilomycin (10, 25 and 50nM) in growth media.  Everything else was the same (except the cells were 1 passage older). The results are the…

All the data presented below was generated by Alex from Adarza Biosystems, Inc. Introduction: Chemical vapor deposition of 99% APTES (3-Aminopropyltriethoxysilane) on planar silicon chips was accomplished using the new vapor deposition system (YES: Yield Engineering Systems). In addition to…

I’ve been trying to convince myself that the black holes seen in endothelial cells that we’ve been calling vacuoles are actually vacuoles.  The first set of experiments involved staining cells with TAMRA, a fluid-phase marker which should get pinocytosed into…

Recently, I have been trying to pass 5 nm, 10 nm, and 15 nm gold through untreated, carbonized, and ozone/carbonized membranes.  These experiments were done in the pressure cell with fresh samples.  I used a 1:1 dilution of Au stock…

Last week, I used a 6-inch thermocouple wafer to profile the temperature uniformity (outside the susceptor) in the RTP.  I measured four points: center, front, back, and center-right (positions relative to the RTP door e.g. front means closer to the…

Back a year or more ago, we had analyzed the EO flow rates from pnc-Si and determined that the flow was much higher than any other material.  We then went on to try to calculate the zeta potential using the…

Ag/AgCl electrodes are used overwhelmingly in streaming potential and sometimes in electroosmosis.  The benefit of using Ag/AgCl in electroosmosis should be less gas generation and the ability to make an enclosed pump.  Here is some information on my attempt at…

All electrokinetic phenomena are connected by a form of the Helmholtz-Smoluchowski equation.  In the case of electroosmosis, the equation can be written as follows: where v is the velocity (in m/s) of water flow through the pores, epsilon the permittivity,…