I asked Tom to add gold PES separations of NPs to his earlier figure and to evaluate the loss of NPs during the use of these filters compared to pnc-Si. Here is what he came up  … All experiments (including…

This device was built similar to the device featured in the Burns(Zydney) 2000 J. Mem. Sci. paper.  Our device seals in a SepCon formatted pnc-Si chip and allows 2 electrodes to be positioned by the chip.  A feed line pressurizes…

In preparation for Tuesdays meeting Jim asked me to prepare this chart to compare the results of the treated and untreated diffusion experiments from this summer. The chart values given are the percent concentration of the sample filtrate in comparison…

In the previous post it seems that the membrane pores do not significantly retard proteins and that the separation characteristics are due more to the slower diffusion coefficients of the proteins.  However, in certain circumstances, the membrane may provide more…

The goal of my rotation was to create a small, self-contained electroosmosis device that operates on a microliter scale using TEM membranes.  Development of this device went through several phases, picking up where Mark Lifson left off.  One important aspect…

I made a post yesterday showing that in 20mM HEPES, pH7.5, +10mM NaCl solution equivalent amounts of DDM were diffusing through the “treated” and “untreated” SC256 samples. Additionally it appeared that minimal amounts of BSA were diffusing across either sample.…

I took some force curves a while ago to see if there was any difference between wrinkled and taut membranes.  Here, I present only the data with a 15nm ‘trigger’.  The trigger is a user-defined parameter that sets the maximum…

Tweaking simulation was not bringing it any closer to the experimental shown in the last post.  This led me to try the experimental once more.  I set up the same system and used 1:2 dilution of the standard protein mixture.…

Yesterday I posted the results from an experiment testing diffusion of BSA and DDM with salts (10mM) across “treated” and “untreated” SC256 samples. The results posted yesterday showed relatively equal amounts of DDM diffusion in both the “treated” and “untreated”…

In my last post I showed that using buffered solutions instead of Di-water we could obtained similar results with SC256 as we had with SC269. I used 20mM HEPES at pH7.5 with no salts and showed DDM diffusion through both…