Hi all, the technique of UV Ozone bonding (of PDMS to glass, Si or pnc-Si) is now complete, serendipitously. Hsin from Miller lab want to bond PDMS to glass, they tried plasma oxidation treatment and it worked.  The method is…

Here is the figure we talked about in the last meeting. For each specific membrane, I’ve plotted both the experimental permeability and the permeability calculated from the theory. You can see in some cases they are close, but in others…

Follow-up to Pressurized Movable Cutoffs. Wafers 670 and 673 were not holding up in PBS for the 24 hour diffusion experiment duration.  RTP treatment at 800C for 60s enabled chips from both of these wafers to last 24 hours.  Here…

I performed a discoloration experiment with w639 in EGM + 10% FBS.  I put 2 blank chips in the Sepcons membrane side down.  I then placed one Sepcon in a well with EGM alone.  The other Sepcon was placed in…

The Bernhard group wants to use our membranes to separate pieces of DNA.  During their experiments they noticed that some of their samples caused discoloration over the summer.  Since then, I’ve been helping a new student to the project, Paul…

This is a repeat of the previous array experiment, but with a higher NaHCO3 concentration (1.85 mg/mL). Also, since these experiments require a lot of samples, I decided to cut chips off of wafer skeletons.  This one was w622.  As…

Based on last week’s lab meeting, I made a “solution array” to test discoloration.  Specifically, I made 3 solutions of NaHCO3 (0, .23125, .4625, .925 mg/mL) and 3 solutions of NaCl (0.1, 0.5, 1 M).  I placed these non-RTP’ed w648…

Last week, I looked at discoloration with different wafer numbers (639,670,672,683,685,702,703), pre- and post-rebuild.  This experiment was done in the oven at 37C, in PBS and without RTP.   Based on the starting color, w670 and w672 are the thinnest…

I attempted a diffusion study with w685 three days ago. If you were following the comments on the w685 page, you’ll know I had some difficulty pipetting the protein solution into the well. I haven’t had this problem recently, and…