My last cell post involved pictures of HUVECs after 1 day of growth on commercial transwells and pnc-Si Sepcons.  Over the past week, I’ve been growing HUVEC on commercial PC transwells and pnc-Si in the Sepcon format in order to…

I repeated the autoclave experiment from earlier this week, but this time I also used the Sepcon format.  I used wafer 662, which was contaminated with large spots.  This was done in the oven in RPMI-1640 with FBS (no RTP). …

My previous discoloration post showed that chips in the Sepcon format discolored much slower than flat chips.  A flat chip in the same well as a Sepcon chip seemed to accelerate discoloration.  One thing about the Sepcon format that I…

Previous Post on Water Permeability Figure We decided at the last meeting to show water permeability for each membrane used rather than plot for a specific characteristic like porosity.  I did this but there were a few samples that still…

Cellulose Membranes I set up a 24 hour diffusion experiment with Millipore 30kD cellulose membranes as shown in this picture: After 24 hours I collected retentate and filtrate and visualized on the following gel: I didn’t expect to see a…

I’ve been doing some preliminary studies with HUVEC in commercial transwells and on pnc-Si in the Sepcon/transwell hybrid format.  The thinness of pnc-Si will enable better microscopic examination of cells than other membrane systems (if pnc-Si is flat).  I thought it would be…

I ordered some new AC160 tips last week from Asylum and got on the AFM Thursday to test them out.  I think they solved some of the problems I was running into over the last few weeks.  Mainly, I was finally able…

Back a long time ago, I had performed some separations with the aim of showing the usefulness of pnc-Si in hemodialysis. I attempted separations of albumin (an important protein to be kept in the blood) and cytochrome c (used in…