A couple weeks ago, I posted some pictures that showed a non-linear “dose-response” curve for NaHCO3 and discoloration in the incubator (post). I repeated this experiment at 37C in the benchtop oven. These pictures show that there was a…
Category: NRG
Recent Discoloration in other Projects
Bernhard Group: Discoloration was observed during DNA separation experiments. Further study led to these results: No discoloration observed with low pH buffered NaCl. Discoloration with high pH buffered NaCl. Worried that Cl was the culprit, sodium acetate was tested. Discoloration…
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New wafers – discoloration
Since Jess saw discoloration last week within a day in PBS with the new material, I did a couple of discoloration studies this week. I looked at wafers 670 and 673 and compared them to 625, 704 and 680-T. These…
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AFM
I got back on the AFM today to rescan the wafers from yesterday and too scan a wafer that Dave had given me a few weeks ago. The 2 panels are from the height channel, I used the 240 tip…
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AFM retry
I posted some pictures yesterday from AFM last week. I got back onto the AFM today and re-scanned the megasonics cleaned virginia semiconductor wafers. For each image, the top and bottom panels are the height and ZSensor scans, respectively. All…
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Discoloration Study with Bacterial Media
I performed a test study to see the effect of microbial media’s : LB and BHI Broth on the membrane to check for discoloration. LB Broth : Lysogeny broth,Luria broth or Luria-Bertani broth. Obtained from Sigma and made by dissolving…
8 hour diffusion
Because of the recent discoloration problems I observed with 24 hour diffusion using w670 and 673, I decided to try a shorter time point. 673 had a couple more samples to spare, so I tried an 8 hour diffusion experiment. …
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AFM – Megasonics
I got some wafers from Chris last week(?) that had undergone different Megasonics cleaning treatments by the manufacturer. We’re trying to figure out if these treatments are actually cleaning the wafers. I’ve been having trouble getting the AFM to stay…
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Pressurized Movable Cutoffs
While wafers 670 and 673 did not hold up to diffusion experiments, they were strong enough to perform pressurized separations using the pressure cell. Each experiment lasted for 40min, although the average flow was only about 5ul for this duration.…
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Water Permeability Statistics
I’ve processed all the images used in the water permeability studies and found the following statistics from these images: Mean Diameter Mean Area Median Diameter Median Area Porosity Area Weighted Diameter 90th percentile Diameter I calculated the theoretical water permeability…
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