I’m having trouble getting the PDMS to cure on the NC. When I first started these experiments in August, the PDMS seemed to cure on the NC without a problem. Now, when I remove the PDMS + NC from the…
Category: NRG
Original Contact Angle Study
This is the original contact angle study performed using the DeLouise Lab’s Rame-Hart contact angle measurement tool. The entire study was performed on 8/1/06-8/10/06. Observations: Contact angles of non-plasma oxidized samples start ~60 degrees and increase over about 10 hrs…
Posted in NRG
Material changes
There’s been a flurry of activity regarding Jess’ recent discoloration post. Specifically, there is a discussion about the recent materials changes that have taken place due to the rebuilt CVC sputterer. This post is meant to supplement the ongoing materials…
Posted in NRG
Diffusion Separations w/ 670, 673
Wafers 670 and 673 were made to have different cutoffs. I set up diffusion experiments with two chips from each wafer using protein standards. However after removing samples after 24 hours I noticed that both chips from w670 had fully…
Posted in NRG
Pore processing influence on permeability results
I mostly finished re-processing the images with new method of using pore processor. The images of molar flow/porosity dependence with older and newer processed data are below. Older image: New image: They don’t look much different. Also you can not…
Posted in NRG
Negative Assay Follow Up
In yesterday’s post I showed that I was able to obtain good negative adsorption assay results, but there was one major problem. The cellulose and PES actually had higher protein concentrations after the assay. I suggested this was because they…
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Negative Adsorption Assay
The Tecan Nanoquant plate is amazing. For those of you who are unaware, we were able to demo a quartz plate that enabled absorbance measurements with only 2ul drops. It took a little bit for me to figure it out,…
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Tecan sensitivity
I did a quick experiment in order to show everyone what the Tecan allows us to do. I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an…
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BSA detection on NC
Creating an array to hold a pnc-Si wafer to proving to be difficult. The PDMS spreads too rapidly though the NC to form a consistent array for the pnc-Si membrane (and eventually wafer) to rest on. I’m still running different…
Membranes with movable cutoff
Last week, we created a series of wafers that were RTPed at four different temperatures between 900 C and 1000 C. Below is a comparison of the 900 C (left) vs. 1000 C RTP (right) wafer. We see that a…
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