The Tecan Nanoquant plate is amazing. For those of you who are unaware, we were able to demo a quartz plate that enabled absorbance measurements with only 2ul drops. It took a little bit for me to figure it out,…
Category: NRG
Tecan sensitivity
I did a quick experiment in order to show everyone what the Tecan allows us to do. I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an…
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BSA detection on NC
Creating an array to hold a pnc-Si wafer to proving to be difficult. The PDMS spreads too rapidly though the NC to form a consistent array for the pnc-Si membrane (and eventually wafer) to rest on. I’m still running different…
Membranes with movable cutoff
Last week, we created a series of wafers that were RTPed at four different temperatures between 900 C and 1000 C. Below is a comparison of the 900 C (left) vs. 1000 C RTP (right) wafer. We see that a…
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Pore Processing
Here is the current method that I am following to find the pore statistics: 1. Select the highest threshold that you can still close with disk of 3 2. Hit majority 3. Close w/ 3 4. — Try open w/…
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w705 Permeability and Separation
W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration…
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Sodium bicarbonate "dose-response"
We’ve been focusing on bicarbonate ions as one of the causes of discoloration. So, I made several sodium bicarbonate solutions and tracked discoloration of non-RTP’ed samples. I made 3.7 mg/mL NaHCO3 (=44mM, this is the concentration in DMEM), 1.85 mg/mL…
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Homemade HBSS test
The last couple of posts showed that homemade HBSS does not cause discoloration, even though commercial HBSS does. I wanted to test if there was something mysteriously special about this solution, so I looked at non-RTP’ed wafer 622 in the…
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Negative Adsorption Assay
This is a retrial of the negative adsorption assay using the Tecan spec/fluorimeter that we have been demo-ing. To perform this assay, I am applying a known concentration to the surface I want to test. After an hour I remove…
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We have the Tecan M200 until Oct. 6th!
Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th. Please come try out your assays on it. Or check sensitivities. Jess or I could probably…
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