What has been accomplished thus far: Able to detect BSA on NC membrane with the use of Ponceau S stain With PDMS array on NC, no BSA leaks through the area covered by PDMS Initial trial done with 10:1 ratio…
Category: NRG
HBSS Components – trial 2
In these discoloration tests, I looked at the components of HBSS. HBSS consists of NaCl, KCl, 0.048 mg/mL Na2HPO4, 0.06 mg/mL KH2PO4, glucose and 0.35 mg/mL NaHCO3. In a previous post, I showed that NaCl, KCl and glucose didn’t cause…
Posted in NRG
Time after RTP – discoloration
For this experiment, I looked into the stability of RTP treatment. Specifically, I tested discoloration rates for chips at different time points after I did RTP. The idea was to see if the beneficial effects of RTP depended on the…
Posted in NRG
Effect of PES Cutoff on "Electro-osmosis" Rate
We have two cutoffs for the Millipore Biomax PES membranes: 50kD and 30kD. I tested the “EO” rates for both of these membranes and plotted them in this figure with standard deviations (3 trials of 50kD and 4 of 30kD).…
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SiO2 deposition – trial 2
Last week, JP deposited 25nm SiO2 for me on wafer 644 (which I cleaned in the UV/Ozone before I gave it to him). Unfortunately, someone broke the E-Beam that day and JP was only able to deposit on 1 side…
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High resolution TEM images from Hitachi
Jamie asked me to post these images of our membranes taken by Dr. Xiao Zhang from Hitachi. They used a 300 kV tool to acquire these micrographs. The spacing between the atoms (striations) tells us the crystallographic orientation; most of…
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w677 Separation
Here are the results of a protein ladder separation with w677. If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that…
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Using JMP7 for Membrane Reliability and Performance
JMP7 statistical software was used to perform a Tukey-Kramer analysis on both burst pressure (psi) and air permeability for membranes fabricated on 200um and 400um wafers. This data has been collected from a spreadsheet that is maintained by Karl and…
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DMEM salt/buffer concentrations
I talked about this yesterday in lab meeting, but here is the data. Since the components of HBSS by themselves did not cause discoloration (HBSS components), I adjusted the concentrations of NaCl, KCl, glucose, Na2HPO4 and NaHCO3 to match that…
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Effect of Active Area on Electroosmosis
Does more active area mean more fluid transported by electroosmosis? To test this I found an ancient wafer (w13) that had different active area geometries. Can someone check and make sure I got these right: Long slit – .1 x…
Posted in NRG
