Fibroblast adhesion experiment was done using poly-lysine coated plastic and teflon coverslips as positive and negative controls repectively. Poly-lysine is a polypeptide that gives the cells a sticky surface to adhere to. Teflon coverslips are used as negative control because…
Category: NRG
Polycarbonate Sheet — substrate material
I worked with Paul this afternoon and finally we highly suspect the raw material used for the prototype diffusion chamber could be Polycarbonate. Paul could not provide direct evidence for the material source since he did not keep any purchase…
Posted in NRG
Question about observation through the plastic substrate of the new diffusion chamber
Attached are the photos taken under DIC and Rhodamine channels, using the new diffusion chamber. I can clearly observe Rhodamine pass to the lower hole of the chamber, under the Rhodamine channel by eye ball. But from what I got…
Posted in NRG
Silica NP sizes
Hybrid silica studies the size of their particles by fluorescence correlation spectroscopy. I used our dynamic light scattering instrument to see what sizes it would get from these particles. Their 45nm particles: Avg center of peak = 46.2 nm Avg…
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Silica NP Separation
Here’s my ‘blog’ post using Google’s Picasa photo album: A few weeks back I received nanoparticles from Ithaca startup company called Hybrid Silica Technologies (HST). They are silica particles that are infused with fluorescent dyes that are generally similar in…
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Flow through wet/dry membranes?
I obtained some perplexing results today that I have re-run multiple times. Ozone treating w504 permits the passage of water in a wet/dry format w/o PVP coating. I ran several experiments with the constant pressure device with water on the…
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Fibroblast Adhesion Update
Four more adhesion experiments were carried out and the results can be seen in the following charts. Based on the average of the four trials it can be elucidated that the initial adhesion (after ~7 hours) is pretty much the…
Adsorption Quantification II
I’ve updated the figure from this post to add new data from oxidized and pre-washed glass samples. We now have possession of teflon material, which I will be testing this week.
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