w565 is permeable to rhodamine. I tested two chips using 100uM rhodamine using a time step of .5 minutes. Click on pictures to see animations. position (1,-1) – While trying to do this first sample, MATLAB crashed and I was…
Category: NRG
R6G diffusion observed through the plastic substrate
The only problem is the diffusion is very fast with defects in there.
Posted in NRG
Desalting with Dialysis Cassettes
One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Two dialysis products made my Thermo/Pierce were tested to develop a protocol and test manufacturer’s claims. These products…
HUVEC Adhesion with controls (repeat)
HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as…
Adsorption Quantification IV
Previously we have worried about the validity of our adsorption data considering we have used it to test both sides of anisotropic membranes that have cutoffs below the size of albumin. Today we came up with the idea that albumin…
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HUVEC Adhesion with Controls
HUVEC adhesion was measured on glass, tissue culture plastic, and pnc-Si, with poly-lysine coated plastic and teflon coverslips as positive and negative controls. Adhesion on pnc-Si was low, this could be due to cells being lost through broken slits or…
Micropump Update
Finally on Friday all the pieces came in that we ordered to connect the peristaltic pump tubing to the 238umOD capillary tubing. This is our first order, after last weeks meeting we ordered a smaller OD capillary tubing (164um) with…
Adsorption Quantification III
UPDATE: Teflon data now include Original Post: Refer back to Part I, and Part II This weekend I ran a bunch of gels to flesh out the adsorption data a bit more. I tested MicroCons, NanoSeps, and teflon and reran…
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pinhole-less membranes
Inspired by JP, we decided to try to etch amorphous (unannealed) wafers this week to see if pinhole density changed. To my surprise, the amorphous membranes had no pinholes! Furthermore, I tried to anneal the membranes after the etch and…
Posted in NRG
COMSOL modeling progress…
Over the last few days I have spent some time learning the basics of the COMSOL modeling software. I have just started to understand the basics and I just wanted to show an example of what this program is capable…
