After posting my observed results from yesterday I came in today to find that one of the segment of the PDMS was strongly bound to the glass cover slip, so strong in fact that trying to remove it caused the…
Category: NRG
Post-etch annealed membranes
Below are images from two samples that were annealed at 950 C and 1050 C post-EDP etch. The lower temp anneal produced a membrane with many features that do not seem to penetrate the nc-Si. There were a few (couple…
Posted in NRG
Using UV Ozone to Bond PDMS to Glass
I talked with Jim last Friday about a second possible dialysis system using PDMS bound to a glass cover slip. I made a batch of PDMS and let it cure at room temperature over the past few days. Jim had…
W565 Air Permeability and Burst Pressure
I tested four samples (-1,1); (-1,2); (-1,3); and (-2,4) for air permeability and burst pressure. The first three samples showed no measurable air permeability for three attempts each. The sample from position (-2,4) did have a very small air permeability…
w565 Rhodamine diffusion
w565 is permeable to rhodamine. I tested two chips using 100uM rhodamine using a time step of .5 minutes. Click on pictures to see animations. position (1,-1) – While trying to do this first sample, MATLAB crashed and I was…
Posted in NRG
R6G diffusion observed through the plastic substrate
The only problem is the diffusion is very fast with defects in there.
Posted in NRG
Desalting with Dialysis Cassettes
One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Two dialysis products made my Thermo/Pierce were tested to develop a protocol and test manufacturer’s claims. These products…
HUVEC Adhesion with controls (repeat)
HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as…
Adsorption Quantification IV
Previously we have worried about the validity of our adsorption data considering we have used it to test both sides of anisotropic membranes that have cutoffs below the size of albumin. Today we came up with the idea that albumin…
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HUVEC Adhesion with Controls
HUVEC adhesion was measured on glass, tissue culture plastic, and pnc-Si, with poly-lysine coated plastic and teflon coverslips as positive and negative controls. Adhesion on pnc-Si was low, this could be due to cells being lost through broken slits or…
