Category: NRG
Flow through wet/dry membranes?
I obtained some perplexing results today that I have re-run multiple times. Ozone treating w504 permits the passage of water in a wet/dry format w/o PVP coating. I ran several experiments with the constant pressure device with water on the…
Fibroblast Adhesion Update
Four more adhesion experiments were carried out and the results can be seen in the following charts. Based on the average of the four trials it can be elucidated that the initial adhesion (after ~7 hours) is pretty much the…
Adsorption Quantification II
I’ve updated the figure from this post to add new data from oxidized and pre-washed glass samples. We now have possession of teflon material, which I will be testing this week.
6/9 Updates on production
Here’s the latest news from the production front: We are still struggling with the pinhole issue. Last week, Chris and JP tried a matrix of conditions to try to eliminate the particle contamination. Efforts included using the CO2 snow cleaner…
Diffusion Stir Cell w microbeads
Mike and I tried to pass 3 um beads mixed with rhodamine through the stir cell to prove that, if the membrane we intact, only the rhodamine would pass through and not the beads. As seen in the pictures below,…
Cell Adhesion Testing 6/3-6/4
Two more adhesion tests were run recently, one on Tuesday and one on Wednesday. For both tests cells were imaged after five hours. Fibroblast trial 3 was the first test where the optimal cell density (assuming 100% of cells are…
Problems with the New Diffusion Chamber
I have tested the new diffusion chamber shown in a yesterday’s post. The idea of our original design works. But there are several problems with this prototype: – Paul used a very thick material to build the clamp, which blocks…
Diffusion Cell
I ran a few tests with rhodamine to see if the fluorescence will transfer across the cell. The problem I keep having is that the stir cell set-up is very sensitive. If the wing nuts are tightened too much the…
Micropump Calibration Curves
The controls of the micropump are in units of frequency, voltage, and wave form, not in flow rates. Based on the manufacturers website they claim that the flow rate has a linear relation with voltage. I took some time to…
