Here’s the latest news from the production front: We are still struggling with the pinhole issue. Last week, Chris and JP tried a matrix of conditions to try to eliminate the particle contamination. Efforts included using the CO2 snow cleaner…
Category: NRG
Diffusion Stir Cell w microbeads
Mike and I tried to pass 3 um beads mixed with rhodamine through the stir cell to prove that, if the membrane we intact, only the rhodamine would pass through and not the beads. As seen in the pictures below,…
Cell Adhesion Testing 6/3-6/4
Two more adhesion tests were run recently, one on Tuesday and one on Wednesday. For both tests cells were imaged after five hours. Fibroblast trial 3 was the first test where the optimal cell density (assuming 100% of cells are…
Problems with the New Diffusion Chamber
I have tested the new diffusion chamber shown in a yesterday’s post. The idea of our original design works. But there are several problems with this prototype: – Paul used a very thick material to build the clamp, which blocks…
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Diffusion Cell
I ran a few tests with rhodamine to see if the fluorescence will transfer across the cell. The problem I keep having is that the stir cell set-up is very sensitive. If the wing nuts are tightened too much the…
Micropump Calibration Curves
The controls of the micropump are in units of frequency, voltage, and wave form, not in flow rates. Based on the manufacturers website they claim that the flow rate has a linear relation with voltage. I took some time to…
Posted in NRG
Capillary Tubing and Adapter
Just wanted to let everyone know that the fused silica capillary tubing and adapter needed to connect them to the pump tubing have been ordered and should be in hopefully by Friday (Monday at the latest).
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New diffusion chamber prototype
Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is…
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pH and Salt test on pncSi
We have been wondering about the reason for discoloration for a while. This could be due to the pH of the solution or the concentration of the salts in the solution. To narrow down our vision on this, first we…
HUVEC and Fibroblast Growth Summary
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are…
