(Transferred from old blog) The above TEM images show that RTP (800C, 5 min) treated samples (from W334) have their pores intact in salt over a period of time! 1M and 2M NaCl solutions were used and TEM samples were…
Category: NRG
RTP Treated Membrane Stability in DMEM
(Transferred from old blog) Wafer 303 samples, both non-RTP and RTP (800C for 5 minutes), were tested in cell media (DMEM supplemented with 10% FBS) or deionized water, and placed in the incubator (37C 5% CO2) or oven (approximately 37C).…
pH Variation of DMEM
(Transferred from old blog) Aim: Determine how quickly DMEM pH changes once removed from the incubator Methods and Materials: • non-sterile complete Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS • 6 and 24 well cell culture plates •…
Dye transport through 501
It took me a little while to figure out how to turn the .avi file into a .gif, but here is an image of the diffusion through 501. Due to a problem with MatLab, it took about 5min before I…
Posted in NRG
W501: Air Permeability and Burst Pressure
Here are the results for air permeability testing of W501. The sample closest to the origin had one very small (very difficult to see on Brian’s scope unless viewing at 100x) pinhole, the middle sample had 3 very small pinholes.…
Posted in NRG
TEM of PVP treated membranes
The unwashed membrane has a definite “film” over it which makes the pores look textured. The MeOH & H2O seems to clear the pores up, but there are some places where there is remaining PVP that sticks to the inside…
Posted in NRG
TEOS vs. Thermal Oxide
This week, we explored the possible differences a thermal oxide making layer had versus a TEOS on membrane morphology. Here’s what we found: The top image shows wafer 410 @ (1,0) and the bottom wafer 503 @ (1,0). Both wafers…
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HUVEC Growth on Si
Over the past five days Anant and I monitored human umbilical vein endothelial cell (HUVEC) proliferation on three substrates: RTP-treated Si samples (both with and without membranes), glass (microscope coverslips), and plastic (6-well tissue culture plates). After the first two…
Protein separations with w415,416
I recently set up 24 hour diffusion separations with the BioRad protein standards. The following gel shows the separations using w415 position (-4,3) and w416 (-1,3). Different cutoffs were observed with the two membranes.
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W415-T and W416-T: Air Permeability and Burst Pressures
I recently tested intact samples from W415-T and W416-T. All samples from W415-T were completely intact except the one located 3.167 units from center, which had one medium pinhole. All samples from W416-T were intact, but a few had irregular…
