This week, we explored the possible differences a thermal oxide making layer had versus a TEOS on membrane morphology. Here’s what we found: The top image shows wafer 410 @ (1,0) and the bottom wafer 503 @ (1,0). Both wafers…
Category: NRG
HUVEC Growth on Si
Over the past five days Anant and I monitored human umbilical vein endothelial cell (HUVEC) proliferation on three substrates: RTP-treated Si samples (both with and without membranes), glass (microscope coverslips), and plastic (6-well tissue culture plates). After the first two…
Protein separations with w415,416
I recently set up 24 hour diffusion separations with the BioRad protein standards. The following gel shows the separations using w415 position (-4,3) and w416 (-1,3). Different cutoffs were observed with the two membranes.
Posted in NRG
W415-T and W416-T: Air Permeability and Burst Pressures
I recently tested intact samples from W415-T and W416-T. All samples from W415-T were completely intact except the one located 3.167 units from center, which had one medium pinhole. All samples from W416-T were intact, but a few had irregular…
TEM of PVP coated membranes
Tom prepared two PVP coated TEM samples for me to image today. One of the samples was washed with dH20 and the other left untouched. Below is a series of three images, from left to right: no PVP, PVP no…
Posted in NRG
Adjusted Track-Etched Permeability Plots
I recalculated the permeability of the track-etched membranes using Poiseuille’s Law this time assuming that the pore diameters specified by Sterlitech are smaller then the actual pore sizes. For the first recalculation I assumed that the “real pores” are 20%…
Protein Ladder Separations
We’ve recently put together our own protein ladder, which contains myosin heavy chain, beta-galactosidase, phosphorylase b, IgG, albumin, and cytochrome c. This is my first attempt running this ladder on w398, one of the new pinhole free membranes. From the…
Posted in NRG
Membranes on STEM
Chris and I got to play on the new Zeiss scanning transmission electron microscope (STEM) today (learn about STEM here). We didn’t get to spend a lot of time on it, but here are some quick images we snapped. wafer…
Posted in NRG
Wettability (with PVP) !!!
The new bottle of PVP (polyvinylpyrrolidone) sitting on the shelf was calling my name this morning. I apologize that this post is long, but its pretty exciting – I think we have half-solved the wettability problem. According to MSDS sheets,…
Posted in NRG
Cell Labeling on Si
Below is an image of HUVEC grown on RTP treated W303 for 72 hours. The cells were seeded at high density and therefore reached confluence prior to day 3. The first row shows the membrane area of the sample; the…
