Tom prepared two PVP coated TEM samples for me to image today. One of the samples was washed with dH20 and the other left untouched. Below is a series of three images, from left to right: no PVP, PVP no…
Category: NRG
Adjusted Track-Etched Permeability Plots
I recalculated the permeability of the track-etched membranes using Poiseuille’s Law this time assuming that the pore diameters specified by Sterlitech are smaller then the actual pore sizes. For the first recalculation I assumed that the “real pores” are 20%…
Protein Ladder Separations
We’ve recently put together our own protein ladder, which contains myosin heavy chain, beta-galactosidase, phosphorylase b, IgG, albumin, and cytochrome c. This is my first attempt running this ladder on w398, one of the new pinhole free membranes. From the…
Posted in NRG
Membranes on STEM
Chris and I got to play on the new Zeiss scanning transmission electron microscope (STEM) today (learn about STEM here). We didn’t get to spend a lot of time on it, but here are some quick images we snapped. wafer…
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Wettability (with PVP) !!!
The new bottle of PVP (polyvinylpyrrolidone) sitting on the shelf was calling my name this morning. I apologize that this post is long, but its pretty exciting – I think we have half-solved the wettability problem. According to MSDS sheets,…
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Cell Labeling on Si
Below is an image of HUVEC grown on RTP treated W303 for 72 hours. The cells were seeded at high density and therefore reached confluence prior to day 3. The first row shows the membrane area of the sample; the…
Wafer 416-T
Wafer 416-T is a TEOS wafer annealed at 900 C. Pinhole density is relatively low. left: (1,0) @ 35kx; right (0,4) @ 35kx left: (1,0) @ 50kx; right (0,4) @ 50kx
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SEM images of vertically cut track-etched membranes
TE membranes were first embedded in plastic material and then these samples were cut with microtome and imaged. The images below are of the 30nm and 80nm pore diameter membranes. 30nm ones came out better. It looks like the diameter…
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Wafer 398
Wafer 398 was deposited on 2/14/2008 but just etched yesterday in the new cell (it’s a beauty esp. when you compare it to the old one). I’ve etched several wafers now in the new cell and none of the membranes…
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Wafer 415-T
Wafer 415-T has been delivered to the lab. It was processed with TEOS and annealed at 950 C. There are a few usable samples, especially along the edge of the wafer. Pinhole density increases as you move towards the center.…
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