The following changes were made in this adsorption study: The MicroCons/NanoSeps were not spun. We will just characterize the differences between the materials. The pnc-Si membranes were assembled into SepCons because of the potential adsorption to the plastic surfaces. Mass…
Category: NRG
Pinhole Study: W510
To improve our knowledge of pinhole formation I have started counting pinholes on W510, W512, and W513. I also am playing close attention to areas surrounding the samples that have been completely etched, to see if this correlates to pinhole…
Wafers 510, 511 & 513
Last week I etched three wafers of different silicon thicknesses (10 nm, 15 nm, 20 nm). All the wafers had a TEOS mask with the standard three layer sputtered film on the front. Not that surprising is the fact that…
Posted in NRG
Sample buffer adsorption assay with pnc-Si
Previously I have shown that sample buffer can be used to lift adsorbed proteins off polymer membranes, which can then be imaged on a gel. I have used a similar procedure to assay adsorption on pnc-Si membranes. I used 4…
Posted in NRG
HUVEC Growth on Si: Take 2 and 3
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test.…
Diffusion Cell New Designs
Here are two new designs for the new diffusion cells. Design_2 could be a better solution as it separates the two connections and uses screws to make the wire contacts to outside circuit. Please feed back any ideas to improve…
Etch cell troubleshooting
As many of you know, we have been having a lot of trouble with the new etch cell and have not been able to produce high quality membranes. Yesterday, we discovered that the stainless steel used to fabricate the new…
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Separations with Millipore and Pall Membranes
In anticipation of the pnc-Si separation paper, I have performed a couple of preliminary separations using NanoSeps and MicroCons. I applied 200ul of 1:20 brain extract ( ~.9mg/mL) to 100k NanoSeps and MicroCons, and I spun the samples for 3…
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Adsorption to polymer membranes
Previously we have studied protein adsorption to pnc-Si membranes by TEM and fluorescence. Neither of these methods will work with polymer membranes. Polymer membranes are too thick to view using TEM. Polymer membranes also autofluoresce creating a lot of noise…
Posted in NRG
Incubator versus Benchtop
(Transferred from old blog) From the above discoloration array it can be inferred that the RTP treated samples in the incubator are discoloring in much slower rate than the ones on the benchtop. Thus, incubator conditions prevent the chips from…
