Introduction In the Glading Lab we study Cerebral Cavernous Malformations (CCMs). In CCMs, brain capillaries develop enlarged lumens and a disrupted blood-brain barrier. The presence of a mutation in KRIT1 causes loss of KRIT1 protein function in the endothelial cells.…
Tag: permeability
Monolayer Culture of mouse CMECs on μSIM A-Line Devices
Introduction In the Glading Lab we study a rare disease called Cerebral Cavernous Malformations (CCMs). In this disease, brain capillaries develop greatly enlarged lumens and a disrupted blood-brain barrier. A genetic cause of the disease is the presence of a…
Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 2: Experimental Validation)
This is the second installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position…
Permeance and Hydraulic Permeability of ~500 nm pores in 5-slot chips
Permeance Testing Sepcons were built with 500 nm 5-slot chips. Permeance tests were performed on Samples A-C. Nitrogen gas was pushed through the membrane at a given pressure, and the height on the flow meter tube (green graduated column pictured…
Permeability Graphs for BSA Separations with Variable-Active-Area Sepcons Show Flow is Independent of Fractional Active Area
What I had in my two previous posts described as permeability graphs were in fact total volume passed/ total time passed graphs: What I thought I saw was that cake layer formation was dependent on the filter geometry. There is…
Pressurized Gold Separations – Part 3
Dan Mendelson performed gold separations using wafer 255 and has shown that this wafer has a cutoff for gold near 25 nm. Below I have plotted the data of filtrate gold concentration as a function of the feed concentration for…
W509X Burst Pressure and Air Permeability
W509X, with single 100um by 1000um slits, was tested for air permeability and burst pressure. Prior to testing, I inspected all membranes for pinholes.; every sample had pinholes on the membrane perimeter, as shown below. The first two images are…
Diffusion Stir Cell w microbeads
Mike and I tried to pass 3 um beads mixed with rhodamine through the stir cell to prove that, if the membrane we intact, only the rhodamine would pass through and not the beads. As seen in the pictures below,…
Adjusted Track-Etched Permeability Plots
I recalculated the permeability of the track-etched membranes using Poiseuille’s Law this time assuming that the pore diameters specified by Sterlitech are smaller then the actual pore sizes. For the first recalculation I assumed that the “real pores” are 20%…
