last week I ran some tests using a single 100K microcon filter; this week I preformed essentially the same tests but instead of testing one filter over the entire pressure range I used a new filter at each pressure. I…

Permeability data first:Wafer 310r = 4.3 units from centern = 32.0 mg/mL cytochrome C in 1x PBSmean permeability = 27-33 uL/(min-cm2-psi) *** corrected to account for (2) 115x2014um slits[The range is due to variations in measurement and total accounting for…

Jim’s post inspired me to think about wetting of nanochannels. We raised this question before – Do we wet the channel in our membrane when water is applied to only one side? My guess was yes because of our success…

The following contribution comes from Jan Hoh – a Professor of Physiology at Hopkins, an invited speaker to BME in December, and a potential future collaborator. Jan sent his thoughts on why it is impossible to get flow through our…

Previously I had posted a chart depicting the permeabilities of SepCon membranes from w310. I went back and noticed some errors in the equations. Here is an updated version of that chart. Also included is a sample where I used…

Last week I performed spins with SepCons from w310. I tracked permeability and tried to perform some separations with proteins. w310 had high air permeability (link), but from my diffusion data it looked looked like the cutoff was possibly between…

I ran the same 100K microcon system through numerous pressure ranges and put together the spread of hydraulic permeability results.These results show that the hydraulic permeability of the microcon membranes decrease with increasing pressure. Once I finished running the tests…

I think the consistently low flow rates are a real bottleneck for all our fluid based experiments and must be overcome. On the production side, we are really pushing the limits of our capabilities to produce the current membrane geometry,…

This is some really preliminary data that I hope to add to in the next couple of days. Yesterday I prepared 4 SepCon samples from wafer 310. I spun them at 3.3 psi for 2 hours with just PBS. I…

Using Mike B.’s MATLAB script, I was able to generate some histograms and stats for wafer 187, 310, 319 and 320. I excluded the “small pore background”, as I do not believe these features go all the way through the…