Document: Ian’s Presentation Thanks to Ian Krout for the experiment and the writeup, which I have organized here. The purpose of this set-up was to develop a tool to melt plastic particles collected on the silicon nanomembranes membranes. Due to…
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Integration of in situ Heating for Microplastic Melting Assay on Silicon Nanomembranes
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Thickness and pore-size selectivity of neutrophil transmigration: Which membrane parameters matter and how would they affect transmigration?
Continuing the studies on neutrophil transmigration in devices with embedded parylene membranes of different pore sizes and thicknesses, three pore sizes (1.0 um, 1.4 um and 1.7 um) and two thicknesses (0.7 um and 1.2 um) were used for…
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Discontinuous surfaces reduce nuclear YAP localization through diminished cell spreading in ADSCs
We previously explored cellular behavior on porous substrates and discontinuous substrates. We have seen differences in cell migration and cell-substrate interactions in the form of altered f-actin polymerization, focal adhesion formation, and fibronectin fibrillogenesis on these discontinuous substrates. We were…
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Alec’s Proposal Exam Documents
Here are my proposal exam materials for reference. Proposal Document: Proposal_Salminen Proposal Presentation (No Animations or Videos): Proposal Presentation_No Animations
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Ian Krout Rotation: Microplastic Filtration and Raman Analysis
Word Document: Blog Write Up 4.8.19 Over the past two weeks I have been working on creating a protocol to eliminate the organic debris from environmental water samples, leaving only microplastics behind on the silicon filters. The goal for this…
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The Completion of the First Stage of the Gauntlet (Knocking Aim 1 Out of the Park)
This post has been a long time coming, but I have finally (essentially) finished all the work for Aim 1 of my thesis. (Credit to Dan for helping with part of it and getting a thesis along the way, see…
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Cells Growth in Different Media
The goal of this set of experiments was to study the growth rate of cells in 3 different media; 1. Complete Media 2. Exosome Free Media 3. Serum Free Media. We studied the effect of media on both ADSCs and…
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Critical Flux of Ultrathin Silicon Membranes in Tangential Flow Filtration of Protein Solutions
This post is an accumulation of the results obtained from my research thus far. To recap, my intent (for my masters thesis) is to provide context for critical flux optimization in NPN microfluidic devices, particle capture, and compare critical flux…
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Microfluidic Permeability Assay for In Situ Monitoring of Endothelial Barrier Properties
Introduction As part of my thesis, I have been working on developing an assay for measuring vascular barrier properties within our microfluidic devices. Traditionally, small molecule permeability measurements are performed on cells plated in a Transwell cup. The basic protocol…
[Rotation] HUVEC Cell Culturing on Aline and SiMPore Devices
Introduction Aline and SiMPore are producing devices at much higher manufacturing rates than we were capable of in the McGrath lab. In order to verify that these manufactured devices are capable of growing cells and allowing for cell survival, live/dead…
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