The goal of this set of experiments was to study the growth rate of cells in 3 different media; 1. Complete Media 2. Exosome Free Media 3. Serum Free Media. We studied the effect of media on both ADSCs and…
Category: NRG
Critical Flux of Ultrathin Silicon Membranes in Tangential Flow Filtration of Protein Solutions
This post is an accumulation of the results obtained from my research thus far. To recap, my intent (for my masters thesis) is to provide context for critical flux optimization in NPN microfluidic devices, particle capture, and compare critical flux…
Posted in NRG
Microfluidic Permeability Assay for In Situ Monitoring of Endothelial Barrier Properties
Introduction As part of my thesis, I have been working on developing an assay for measuring vascular barrier properties within our microfluidic devices. Traditionally, small molecule permeability measurements are performed on cells plated in a Transwell cup. The basic protocol…
[Rotation] HUVEC Cell Culturing on Aline and SiMPore Devices
Introduction Aline and SiMPore are producing devices at much higher manufacturing rates than we were capable of in the McGrath lab. In order to verify that these manufactured devices are capable of growing cells and allowing for cell survival, live/dead…
Posted in NRG
BdEC/T24 co-culture: invasion assay (preliminary)
Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24…
Posted in NRG
BdEC/T24 co-culture: wound healing
Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24…
Posted in NRG
Automatic WEKA Segmentation of Microdebris on 8000 nm Slot Nanomembranes
One advantage of our slot nanomembranes is that we can readily image microparticulate debris with simple light microscopy. With that in mind, it should be straightforward to have some image processing along with our microspectroscopy to separate out different particles…
Posted in NRG
Neutrophil transmigration through Parylene membranes
Tom came up with this idea of using these membrane with precise thickness and pore size control to obtain a critical thickness and pore size, beyond which neutrophils cannot go transmigrate. N-Formylmethionyl-leucyl-phenylalanine (fMLP) was used in the lower channel to…
Posted in NRG
Highly porous Parylene membranes with precise control over pore size using a single mask
Parylene-C is a biocompatible, inert, FDA-approved and nondegradable material, which can be used to fabricate mechanically robust thin membranes. Parylene is used in the medical device industry for coating implantable devices and in the microelectronics industry as insulator. Furthermore, it…
Posted in NRG
Latest Update on Wound Healing Study
Introduction: Fibroblasts: Dermal fibroblasts are cells within the dermis layer of skin which are responsible for generating connective tissue and allowing the skin to recover from injury. A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the…
Posted in NRG
