Introduction In a previous post I introduced the concept of endothelium apicobasal polarity and the potential to utilize this concept for future sepsis drug development: Endothelium Apicobasal Polarity Regulates the Innate Immune Response In Vitro [In Progress] In this post,…
Category: NRG
Potassium Sensor Development
As previously stated in my initial post (found here https://trace-bmps.org/nanomembranes-in-potassium-ion-selective-electrodes), I am in the process of developing a potassium Ion Selective Electrode (ISE) Sensor using our chips. Along the way I have gathered all sorts of curious but interesting data,…
ISEV-MRS 2019 Highlights
Hello Everyone, Here is a summary of my trip to Nashville to learn about extracellular vesicles in cancer and present my work on isolation of nanoparticles/EVs using ultrathin nanomembranes. Main points of the conference: This conference was about EVs in…
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Separation of Extracellular Vesicles using Ultracentrifuge
Hi everyone, One of the objectives in the last aim of my PhD is to investigate the effect of soluble factors secreted by adipose derived stem cells (ADSCs) on wound recovery of fibroblasts (FBs). We have categorized these soluble factors…
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To normalize or not to normalize
Hello everyone! this post describes how we are going about determining the size cut-off of the membranes we make through nanosphere lithography. These are 120 nm thick silicon nitride membranes with controlled pore size between 320 and 80 nm. A…
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Assessing the Capacity of Ultrathin Microporous Membranes
As part of my thesis (and a tie-in to the microplastics research our lab has been conducting) I’ve been working on creating a measure of membrane capacity. A robust way to do this involves using microporous chips and polystyrene beads.…
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Modeling leukocyte transmigration thorough a blood-brain barrier model
This post is dedicated to our ongoing collaboration with Dr. Marchi at Centre national de la recherche scientifique (CNRS) in France. The basic concept of the project is simple (Fig.1) and involves attaching a microporous membrane on top a 3D…
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Tensile Mechanical Testing of Ultrathin membranes
As part of Aim 1 of my PhD, I am looking into mechanical characterization of ultrathin membranes to explore the effects of porosity on the structural integrity and determine how porous we can go with a porous membrane, and last…
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Preliminary Testing for Transwell Cancer Exosome Co-culture Studies
Last time this project was discussed at NRG, Henry was the project lead (we miss you, Henry). He was observing bladder epithelial cell (BdEC) invasion and migration in co-culture with T24 bladder cancer cells. Here are links to his previous…
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Kilean’s JMS Paper Figures
Hi All, This post is more of a dump of figures that I have put together for Dan’s and my upcoming Journal of Membrane Science manuscript. I have included figures and captions, so any feedback would be greatly appreciated.
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