Category: NRG

PLL-g-PEG patterning on SiO2 layer

As part of my thesis, I am trying to get a non-fouling pattern on a substrate which resembles our porous membranes to investigate cell behavior including cell adhesion and migration and to compare with our previous results which was obtained

Posted in NRG

Optimizing Urea Clearance via Flow Rate

In my previous post, our urea clearance for the single pass experiments was found to be ~140 ml/min/m^2, which was similar to Fissell/Roy’s value except we used a flow rate of 1uL/min whereas they used a flow rate of 10

Posted in NRG

Particle Tracking in COMSOL Multiphysics

This is a continuation of my post from last time where I looked at modeling actual pore geometries in COMSOL. In this post, I explored the new feature that we have with COMSOL that is the Particle Tracing Module. This

Posted in NRG

eLIFE submission: A silicon nanomembrane-based in vitro platform to visualize immune cell trafficking across the human blood-brain barrier

We have been collaborating with Britta Engelhardt’s group at the University of Bern in Switzerland since Tejas and I met them at the CNS Barriers conference in Paris in 2015. They are interested in studying live T-cell transmigration across human

Posted in NRG

1257 NPN SERS Wafer Partial Etch Study

We manufactured a wafer specifically for SERS experiments in the hope of recapturing larger pore distributions/pore densities that we observed in some of the prototype runs of NPN. Natively, it’s a little thicker, and a little less porous than the

Posted in NRG

Track Etched and NPN Experiments Comparison

Hello Everyone, As you probably know, i have been working on calibration curves for NPN and TE membranes and comparison of these two sets of experiments with each other. This post is all about how i made the calibration curves

Posted in NRG

Generating COMSOL Simulations of Actual Pore Geometries

As Greg posted last time, he and I have been working on a manuscript for all of the tomography stuff that we have done that we are going to hopefully submit this week. In that paper, we covered all the

Posted in NRG

COMSOL model of SiMPore Liftoff device

I drew an approximation of the SiMPore liftoff device and ran the COMSOL model with 1 mL/min urea fluid flow in the upper channels and 2 mL/min dialysate in the bottom channels. With this initial model, the urea was cleared

Posted in NRG

Instantaneous Clearance per unit area – Animal Study

We’ve calculated our instantaneous clearance numbers for the benchtop studies that use sepcon chips. Even calculating per unit area, our chips are not clearing much volume in these experiments. I believe this is due to the very low flow rate

Posted in Knowledge, NRG

TEM Tomography for the Identification of Nanopores in Silicon Nanomembranes

Kilean and I are working on submitting a paper to Membranes on some of our electron tomography work. Here’s some of the work that we’ve done so far: Tomography Paper V2_KL

Posted in NRG
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