What is it? A device to remove bubbles from microfluidic lines.  Air and liquid enter on one side and only the liquid leaves.  Our debubbler is disposable, cheap (<$0.50) and easy to make in the lab. Why use a debubbler?…

Hello Everyone, So Now that we have NanoSight in our lab, i decided to run some experiments with different conditions of media and compare the number of particles in each media. We designed this set of experiments like: Water DMEM…

Hello Everyone, So i decided to do some calculations on our 2 different sets of experiments (Track Etched and NPN) and compare the applied and the required force of them, in addition to making sure that we are applying enough…

Abstract: In this project, we want to explore the possibility of detecting molecules in nanopores by building waveguides upon a silicon nanomembrane chip. The first experiment is performed by sending 650nm laser light onto our waveguides and detecting the light…

As part of my thesis, I am trying to get a non-fouling pattern on a substrate which resembles our porous membranes to investigate cell behavior including cell adhesion and migration and to compare with our previous results which was obtained…

In my previous post, our urea clearance for the single pass experiments was found to be ~140 ml/min/m^2, which was similar to Fissell/Roy’s value except we used a flow rate of 1uL/min whereas they used a flow rate of 10…

This is a continuation of my post from last time where I looked at modeling actual pore geometries in COMSOL. In this post, I explored the new feature that we have with COMSOL that is the Particle Tracing Module. This…

We have been collaborating with Britta Engelhardt’s group at the University of Bern in Switzerland since Tejas and I met them at the CNS Barriers conference in Paris in 2015. They are interested in studying live T-cell transmigration across human…

We manufactured a wafer specifically for SERS experiments in the hope of recapturing larger pore distributions/pore densities that we observed in some of the prototype runs of NPN. Natively, it’s a little thicker, and a little less porous than the…

Hello Everyone, As you probably know, i have been working on calibration curves for NPN and TE membranes and comparison of these two sets of experiments with each other. This post is all about how i made the calibration curves…