As Greg posted last time, he and I have been working on a manuscript for all of the tomography stuff that we have done that we are going to hopefully submit this week. In that paper, we covered all the…

I drew an approximation of the SiMPore liftoff device and ran the COMSOL model with 1 mL/min urea fluid flow in the upper channels and 2 mL/min dialysate in the bottom channels. With this initial model, the urea was cleared…

Kilean and I are working on submitting a paper to Membranes on some of our electron tomography work. Here’s some of the work that we’ve done so far: Tomography Paper V2_KL

Introduction As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress. Methods HUVECs (P3,4,5) were seed within our mimetic and left to…

We have a wafer that did not clear 500 nm pores (400 nm thick silicon nitride, #4682). There are about 230 chips that could be useful if they were etched a little. I used a SF6 based recipe (15 sccms…

Microdialysis: Separation of BSA and Cyto C For the past few months I have been studying how well the 75 nm NPN-O membranes are at clearing cyto c while retaining BSA with and without the presence of PEG coating. Additionally,…

So, I have been working with a 4-probe TEER measurement system to record the changes in endothelial permeability during neutrophil extravasation and other biochemical assays such as thrombin treatment and TNF stimulation. 4-probe systems are sensitive because they don’t suffer from…

As the first phase of my thesis, I have been working on parylene C membranes as potential easy-to-make thin polymeric membranes for blood brain barrier models following Dr. Carter’s works. The initial goal is to create membranes with different thicknesses…