I have had a chance to play with some of the chips from Lot 1278, specifically the design with the 2 micron slots. Results are a bit of a mixed bag. Wetting these chips continues to be a challenge. My…

Introduction Since development, much μ-NPN work has been focused on observing cell adherence under flow, cell morphology on deferentially spaced pores, and neutrophil transmigration. This post will detail the entirety of my work with these membranes to date, and plans going…

The purpose of this device is to make uniform and straight scratch wounds in a 96 and 24 well plate for migration experiments. Previously, scratch wounds were made by hand using a p-10 pipette tip by scraping the tip in…

For this post, Henry and I collected data for hemolysis and NTA sizing of soluble graphene oxide (GO).   Hemolysis– prior work on the hemolysis testing of immobilized GO The purpose of the hemolysis experiment was to confirm that immobilized…

For my work with Kyle and Vincent in Ottawa, I wanted to showcase more of the interesting configurations of DNA rather than simply passing the DNA through the nanomembrane. DNA Dual End Blockage and Clearage:   DNA Flossing:   DNA…

Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes  for migration of FBs and eventually closure (healing) of the wound and second effect of…

What is it? A device to remove bubbles from microfluidic lines.  Air and liquid enter on one side and only the liquid leaves.  Our debubbler is disposable, cheap (<$0.50) and easy to make in the lab. Why use a debubbler?…

Hello Everyone, So Now that we have NanoSight in our lab, i decided to run some experiments with different conditions of media and compare the number of particles in each media. We designed this set of experiments like: Water DMEM…

Hello Everyone, So i decided to do some calculations on our 2 different sets of experiments (Track Etched and NPN) and compare the applied and the required force of them, in addition to making sure that we are applying enough…

Abstract: In this project, we want to explore the possibility of detecting molecules in nanopores by building waveguides upon a silicon nanomembrane chip. The first experiment is performed by sending 650nm laser light onto our waveguides and detecting the light…