Introduction: Over the past few weeks I’ve begun working on a project that deals specifically with characterizing tangential flow filtration with our labs ultra-thin membranes. Essentially, I’m working on understanding the advantages our membranes have over conventional/thicker ones by looking…

Hello Everyone, I’ve been working on exosome labelling and trying to characterize it by NTA. So we read a paper published a month ago, explaining the presence of PKH nanoparticles as false positive results in internalization studies. They studied four…

Previously, we showed that our method of pll-peg coating is effective for creating non-fouling substrate which can prevent protein adsorbtion, and this method can be used to create a non-fouling pattern on the surface. In the next step, we wanted…

In this article we’ll share some of our preliminary results regarding the parameters of the NPN device. We will also be making some recommendations for further tuning of the design to specific tasks. We will be comparing one of the…

I have had a chance to play with some of the chips from Lot 1278, specifically the design with the 2 micron slots. Results are a bit of a mixed bag. Wetting these chips continues to be a challenge. My…

Introduction Since development, much μ-NPN work has been focused on observing cell adherence under flow, cell morphology on deferentially spaced pores, and neutrophil transmigration. This post will detail the entirety of my work with these membranes to date, and plans going…

The purpose of this device is to make uniform and straight scratch wounds in a 96 and 24 well plate for migration experiments. Previously, scratch wounds were made by hand using a p-10 pipette tip by scraping the tip in…

For this post, Henry and I collected data for hemolysis and NTA sizing of soluble graphene oxide (GO).   Hemolysis– prior work on the hemolysis testing of immobilized GO The purpose of the hemolysis experiment was to confirm that immobilized…

For my work with Kyle and Vincent in Ottawa, I wanted to showcase more of the interesting configurations of DNA rather than simply passing the DNA through the nanomembrane. DNA Dual End Blockage and Clearage:   DNA Flossing:   DNA…

Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes  for migration of FBs and eventually closure (healing) of the wound and second effect of…